Functional Genomics of the Interactions of Tomato and Pseudomonas syringae pv tomato DC3000

在了解植物与微生物相互作用的分子基础方面取得了快速进展。番茄在这项研究中一直很重要，因为它的遗传易感性、经济价值，以及许多折磨它的疾病的成本。这项工作的核心是丁香假单胞菌pv番茄DC3000与番茄的基因间相互作用。DC3000已成为植物分子病理学研究的一个重要模型，因为它具有遗传易感性，因为它也是拟南芥的一种病原体，因为它与植物的相互作用似乎代表了许多常见的细菌和真菌病原体。另一个使DC3000在实验上具有吸引力的因素是，有证据表明其寄生能力主要基于（未知数量）III型分泌系统注入植物细胞的效应蛋白。这些效应物的鉴定将为有序地解剖潜在致病性的分子过程提供基础，这些蛋白质可能已经进化到精细地改变植物基因表达和代谢，可能是植物生物学家新工具的来源。因此，本项目的首要目标是由基因组研究所对DC3000的全基因组进行测序，并基于基因组搜索编码效应蛋白和其他潜在毒力因子的基因。随后的目标包括微阵列分析，以确定DC3000和番茄中可能在相互作用中重要的基因和调控，以及开发一系列基于绿色荧光蛋白的工具，用于监测细菌-植物相互作用期间活植物组织中的分子/细胞事件。由于DC3000与番茄（和拟南芥）的相互作用涉及高度定位的细胞事件，似乎是高度多因子的，依赖于冗余因子，这些新工具将对发病机制和防御的功能基因组分析至关重要。该项目的其他重要目标包括建立一个面向社区的功能基因组注释网站和数据库，该网站和数据库将服务于更广泛的研究社区，并为涉及高中生的教育推广活动提供核心内容。项目的主要成果是p.s. tomato DC3000的完整基因组序列、细胞生物学指示植物和细菌突变体。基因组序列草图可通过href=www.tigr.org/tdb/mdb/mdbinprogress.html www.tigr.org/tdb/mdb/mdbinprogress.html /a获得，效应基因坐标和其他资源可通过项目网站a href=http://pseudomonas-syringae.org http://pseudomonas-syringae.org a/获得

Rapid progress has been made in understanding the molecular basis of plant-microbe interactions. Tomato has been important in that research because of its genetic tractability, its economic value, and the cost of the many diseases that afflict it. A centerpiece of that work has been the gene-for-gene interactions of Pseudomonas syringae pv tomato DC3000 and tomato. DC3000 has emerged as an important model in molecular plant pathology because of its genetic tractability, because it also is a pathogen of Arabidopsis, and because its interactions with plants appear representative of many prevalent bacterial and fungal pathogens. An additional factor that makes DC3000 experimentally attractive is the evidence that its parasitic abilities are largely based on (an unknown number of) effector proteins that are injected into plant cells by the type III secretion system. Identification of these effectors will provide the basis for orderly dissection of the molecular processes underlying pathogenicity, and these proteins, which presumably have evolved to exquisitely alter plant gene expression and metabolism, are a likely source of new tools for plant biologists. Consequently, the first objective of this project is the sequencing of the complete genome of DC3000 by The Institute for Genomic Research and genome-based searches for genes encoding effector proteins and other potential virulence factors. Subsequent objectives involve microarray analyses to identify genes and regulons in both DC3000 and tomato that are likely to be important in the interaction and the development of a series of green fluorescent protein-based tools for monitoring molecular/cellular events in living plant tissues during bacterium-plant interactions. Because DC3000 interactions with tomato (and Arabidopsis) involve highly localized cellular events and appear to be highly multifactorial and dependent on redundant factors, these new tools will be essential for a functional genomic analysis of pathogenesis and defense. Other important objectives of the project involve the establishment of a community-oriented functional genomics annotation web site and database that will serve the wider research community and also provide the centerpiece for educational outreach activities involving high school students. DeliverablesThe key deliverables for the project are the complete genomic sequence of P. s. tomato DC3000, cell biology indicator plants, and bacterial mutants. The draft sequence of the genome is available through a href=www.tigr.org/tdb/mdb/mdbinprogress.html www.tigr.org/tdb/mdb/mdbinprogress.html /a, effector gene coordinates and other resources are available through the project web site a href=http://pseudomonas-syringae.org http://pseudomonas-syringae.org a/