Function of Juvenile Hormone Esterase Binding Protein in Insects

保幼激素酯酶（JHE）有助于调节保幼激素的滴度，从而调节昆虫幼虫的发育。JHE通过维持适当血淋巴组成的红细胞从血淋巴中主动清除，并在溶酶体中降解。一种结合蛋白P29与红细胞中的JHE结合，似乎对这些细胞中JHE的适当加工很重要。Bonning博士旨在确定P29影响JHE贩运和降解的机制。为此，她将使用烟草天蛾Manduca sexta和果蝇Drosophila melanogaster进行实验，以确定P29的细胞内位置以及P29与JHE相互作用的位置。然后将通过使用D的突变系来阐明P29相对于JHE的特定功能。黑腹果蝇和RNA干扰，以防止这两种蛋白质的相互作用。将观察P29与JHE结合的破坏对JHE的清除和周转的影响。关于P29的性质和功能的新知识将增强我们对蛋白质运输的了解，并可能促进害虫控制新方法的开发。

Juvenile hormone esterase (JHE) helps to regulate the titer of juvenile hormone and, hence, larval development in insects. JHE is actively removed from hemolymph by athrocytes that maintain appropriate hemolymph composition, and is degraded in lysosomes. A binding protein, P29, binds JHE in athrocytes and appears to be important for appropriate processing of JHE in these cells. Dr. Bonning aims to identify the mechanism(s) by which P29 affects the trafficking and degradation of JHE. To do this she will use both the tobacco hornworm Manduca sexta and the fruit fly Drosophila melanogaster in experiments designed to determine the intracellular location of P29, and the location of interaction of P29 with JHE. The specific functions of P29 with respect to JHE will then be elucidated by using mutant lines of D. melanogaster and RNA interference to prevent the interaction of the two proteins. The effects of disruption of P29 binding to JHE on the clearance and turnover of JHE will be observed. New knowledge about the nature and function of P29 will enhance our knowledge of protein trafficking and could facilitate development of novel approaches to insect pest control.