Acquisition of an Atomic Force Bioscope

0091716 edwarwards该拨款为P.I.购买原子力生物显微镜（AFB）的费用提供部分支持伍兹霍尔海洋研究所（WHOI）的实验室。伍兹霍尔地区的许多其他研究人员，在WHOI或隶属于海洋生物实验室（MBL），也将有机会使用该设备。该AFB将代表伍兹霍尔地区研究人员可用的最高分辨率成像工具。它将被安装在，并与许多研究一起使用，P.I.蔡司Axiovert 100倒置荧光显微镜。这将允许同时和在线荧光和原子力图像收集。空军基地的用途将是多种多样的；它将被用作分析工具和成像系统。在P.I.。AFB将主要用于微生物细胞和矿物表面之间的界面力的定量测定，微生物矿物溶解研究，并结合自然发生的微生物种群的形态和结构分析，同时进行微生物的原位系统发育鉴定。其他研究人员将使用该仪器分析岩石摩擦强度表面的物理和化学性质，确定非哺乳动物脊椎动物的细胞骨架结构和功能，分析离子通道与大型高亲和肽神经毒素的结构相互作用，并在进行伴随的电化学测量时绘制质膜的空间图。本基金由地球科学部和海洋科学部共同资助

0091716EdwardsThis grant provides partial support of the costs of acquiring an Atomic Force Bioscope (AFB) for the P.I.'s laboratory at the Woods Hole Oceanographic Institution (WHOI). Numerous other researchers in the Woods Hole area, at WHOI or affiliated with the Marine Biological Laboratories (MBL), will also have access to this equipment. This AFB will represent the highest resolution imaging tool available to researchers in the Woods Hole area. It will be mounted on, and used in conjunction with for many studies, the P.I.'s existing Zeiss Axiovert 100 inverted fluorescent microscope. This will allow simultaneous and in-line fluorescent and atomic force images to be collected. Uses for the AFB will be quite varied; it will be used both as an analytical tool and an imaging system. In the P.I.'s laboratory, the AFB will be used primarily for quantitative determinations of interfacial forces between microbial cells and mineral surfaces, microbial mineral dissolution studies, and simultaneous in-situ phylogenetic identification of microorganisms combined with morphological and structural analysis of naturally occurring microbial populations. Other researchers will use the instrument to analyze the physical and chemical properties of surfaces that dictate frictional strength of rocks, determine cytoskeletal structures and function in non-mammalian vertebrates, analyze structural interactions of ion channels with large high-affinity peptide neurotoxins, and spatially map plasma membranes while making concomitant electrochemical measurements. Support for this grant is shared between Divisions of Earth and Ocean Sciences.***