Dissolved Organic Phosphorus Cycling in the Coastal Ocean

伍兹霍尔海洋研究所的一位研究人员将参加由沿海海洋过程（CoOP）项目资助的沿海海洋陆架迁移进展（COAST）项目的巡航和实地实验。 该PI参与研究将为项目增加磷成分，从而解决俄勒冈州沿海系统中碳、氮和磷循环的耦合动力学问题。 利用COAST抽样计划提供的高时间和空间分辨率，PI计划开展以下研究：（1）确定溶解性有机磷（DOP）和颗粒有机磷（POP）的三维分布，以及它们与溶解性有机碳（DOC）、颗粒有机碳、溶解性有机氮（DON）分布的关系，颗粒有机氮和其他生物学参数;（2）确定溶解有机库和颗粒有机库的C：N：P比值的动态变化;（3）评估短期（在每次21天巡航的样带重新采样期间）与耦合DOC、DON、DOP的季节变化;以及（4）评估地形对DOP和POP循环和跨大陆架输送的影响。 此外，将通过监测田间样品中DOP酶水解的程度来评价DOP的生物利用度。 关于磷酸盐胁迫的原地浮游植物的营养状况将通过大量和细胞特异性酶标记荧光（ELF）碱性磷酸酶（APase）来确定，浮游植物对诱导或减轻的P限制的物种特异性反应将使用ELF-APase在船上营养修正孵育中进行评估。

ABSTRACTOCE-0119134A researcher from Woods Hole Oceanographic Institution will participate in the cruises and field experiments of the Coastal Ocean Advances in Shelf Transport (COAST) project funded by the Coastal Oceans Processes (CoOP) program. The participation of this PI in the study will add a phosphorus component to the project thereby allowing the coupled dynamics of carbon, nitrogen and phosphorus cycling in the coastal system off Oregon to be resolved. Taking advantage of the high temporal and spatial resolution provided by the COAST sampling plan, the PI plans to carry out the following research: (1) determine the 3-dimensional distributions of dissolved organic phosphorus (DOP) and particulate organic phosphorus (POP), and how they relate to distributions of dissolved organic carbon (DOC), particulate organic carbon, dissolved organic nitrogen (DON), particulate organic nitrogen and other biological parameters; (2) ascertain the dynamic variability of the C:N:P ratios of the dissolved and particulate organic pools; (3) assess the short term (during transect re-sampling on each 21-day cruise) versus seasonal variations in coupled DOC, DON, DOP; and (4) evaluate the effects that topography has on DOP and POP cycling and cross-shelf transport. In addition, DOP bioavailability will be evaluated by monitoring the extent of enzymatic DOP hydrolysis in field samples. The nutritional status of in-situ phytoplankton with regard to phosphate stress will be determined by bulk and cell-specific Enzyme Labeled Fluorescence (ELF) Alkaline phosphatase (APase) and the species-specific response of phytoplankton to induced or alleviated P-limitation will be assessed using ELF-APase in shipboard nutrient amendment incubations.