QSB: Operating Principles for Optimal Functioning

总体目标是阐明代谢和遗传网络的操作和设计原则。两个互补的战略将合并。第一个是小的，模块化的途径，形成了生物化学系统的积木，这些模块的复合材料的理论分析。该分析将阐明酶的动态活化概况，这些酶对代谢物的短暂峰值可用性或代谢物组成的持续变化等需求产生最佳响应。第二个策略将需要在E.大肠杆菌，并以此为实验模型，研究通量和具体的监管干预措施的影响。 因此，该系统有可能提供一个研究从头途径设计的体内实验室。

The overall aim is to elucidate the operating and design principles of metabolic and genetic networks. Two complementary strategies will be merged. The first is a theoretical analysis of small, modular pathways, which form the building blocks of biochemical systems, and of composites of these modules. The analysis will elucidate the dynamic activation profiles of enzymes that yield optimal responses to demands like brief peak availability of a metabolite or sustained change in metabolite composition. The second strategy will entail reconstituting sphingolipid metabolism in E. coli and using this as an experimental model for studying fluxes and the effects of specific regulatory interventions. The system thus has the potential to provide an in vivo laboratory for studying de novo pathway design.