Adaptation of a Microtiter Plate Reader in an Undergraduate Biochemistry Laboratory

在过去的几年中，组合技术使化学的某些方面发生了革命性的变化。实施这种最先进的方法的限制因素之一是需要高通量筛选技术。该方案采用了教育和商业文献中的几个步骤，并使用96孔平板阅读器将其应用到本科化学课程中。学生们监测蛋白质折叠，进行酶动力学，筛选磷酸酶抑制剂文库，并开发ELISA技术与微量滴度板阅读器。这些活动向学生介绍高通量、微量滴度板技术，为大量数据的统计分析提供信息，并增加以研究和发现为基础的实验室活动的能力。此外，图书馆的筛选活动正在进行调整，以便用于少数民族小学生和中学生的外展方案。通过测试和调查问题，通过学生在实验和课堂部分的表现来评估该建议的影响。该提案的结果将通过全国CUR研讨会、出版物和外展活动进行传播。

In the past several years, combinatorial techniques have revolutionized certain aspects of chemistry. One of the limiting factors in the implementation of this state-of-the-art approach is the need for high-throughput screening techniques. This proposal is adapting several procedures from the educational and commercial literature and implementing them into the undergraduate chemistry curriculum using a 96-well plate reader. Students are monitoring protein folding, conducting enzyme kinetics, screening libraries for phosphatase inhibitors, and developing an ELISA technique with the microtiter plate reader. These activities are introducing students to high-throughput, microtiter plate techniques, providing information for a statistical analysis of a large pool of data, and increasing the capacity for research-based and discovery laboratory activities. In addition, the library screening activity is being adapted for use in an outreach program with minority elementary and middle-school students. The impact of this proposal is being evaluated by student performance in both the laboratory and classroom portion of the course via test and survey questions. Dissemination of the results of this proposal will be presented through a national CUR workshop, publications, and outreach activities.