Laboratory Investigations Using Quantitative Microscopy

生物科学（61）该项目通过将数字技术应用于课堂，将定量方法完全整合到脊椎动物显微解剖学课程中。虽然数字成像技术现已广泛使用，但在本科课程中有效使用它受到缺乏现成的协议，实验室材料和本科显微镜实验练习的限制。此外，软件指令组通常是在远离生物实验室的计算机实验室。为了解决这些问题，我们1）开发一个实验室手册，特别强调图像处理和定量方法的显微镜，2）创建生物学意义的调查，应用协议，并从中学生收集数字光和电子显微照片，第三章使用学生生成的图像以及商业上可用的数据集来教授图像处理和定量分析可用的软件（Adobe Photoshop和IPTK插件），以及4）在生物实验室的工作台上使用无线联网的iBook计算机教授图像处理和分析。在课程结束时，学生应该能够为光学和电子显微镜准备脊椎动物组织，捕获数字图像，识别和描述特征组织结构，并在样品之间进行定量比较。

Biological Sciences (61)This project is fully integrating quantitative methods into a course in vertebrate microanatomy by adapting digital technology into the classroom. While digital imaging technology is now widely available, using it effectively in undergraduate courses is limited by a lack of readily available protocols, lab materials, and lab exercises for undergraduate microscopy. In addition, software instruction to groups is usually done in computer labs distant from the biology laboratory. To address these problems, we are 1) developing a laboratory manual with special emphasis on image processing and quantitative methods for microscopy, 2) creating biologically meaningful investigations that apply the protocols, and from which students are collecting digital light and electron micrographs, 3) using the images students generate as well as commercially available datasets to teach image processing and quantitative analysis using readily available software (Adobe Photoshop and IPTK plugins), and 4) teaching image processing and analysis using wireless networked iBook computers at the benches in biology laboratories. By the end of the course, students should be able to prepare vertebrate tissues for light and electron microscopy, capture digital images, identify and describe characteristic tissue structures and make quantitative comparisons between samples.