Nährstoffarme Emers-Fermentation terrestrischer Cyanobakterien zur Produktion biotechnischer Wertstoffe

陆地蓝藻的特点是各种各样的有价值的，这从根本上不同于水生生物。目前，由于还没有栽培技术，这些有价值的资源的开发和大量生产这些代谢物还没有实现。因此，我们开发了一种新型的涌现光生物反应器，在项目的第一阶段，培养这组生物的数量足以处理它们。这种新的涌现式光生物反应器首次为获取陆生蓝藻代谢产物谱开辟了一条新的途径。应用的第二个项目周期的目的是1）验证开发的分析，2）通过计算机辅助方法优化蓝藻贵重物品的生产，以及3）将emerse光生物反应器放大约10倍。除了平衡质量流，该项目旨在模拟生物反应器中的流动动力学。总之，这将允许这种新型的涌现光生物反应器的最终表征。这种开发的光生物反应器是理想的，以满足陆生蓝藻的培养要求。一方面，封装在反应器中的光纤（玻璃棒）用作生物体的定殖表面，另一方面，粘附生长的生物体及其生长区最佳地暴露于所提供的光。此外，集成在反应器外围的气溶胶供应器提供水和营养物，使得能够进行靶微生物的营养物贫乏培养。所建立的几何形状以及过程控制允许高度调整以从陆地蓝细菌生产有价值的培养。在这方面，实验设置有利于诱导生产特定的贵重物品，如通过应用UV-A辐射和干燥以及氮消耗的色素scytonemin。此外，外泌多糖生产的优化是被迫的，而其组成以及其含量将通过改变气溶胶的供应来修改。

Terrestrial cyanobacteria are characterized by a great variety of valuables, which differ fundamentally from aquatic organisms. At the moment, the exploitation of these valuables and the production of larger quantities of these metabolites are not realized because the cultivation technology is unavailable yet. Therefore, we developed a novel type of emerse photobioreactor, within the first period of the project, to cultivate this group of organisms in a quantity sufficient for their processing. For the first time, this novel emerse photobioreactor strikes a new path to access the metabolite spectrum of terrestrial cyanobacteria. The aim of the applied second project period is 1) to validate the developed analytics, 2) to optimize the production of cyanobacterial valuables by computer aided methods, as well as 3) to scale-up the emerse photobioreactor about a factor of ten. Apart from balancing the mass flow, the project aims to model the flow dynamics in the bioreactor. Altogether, this will allow the final characterization of this novel emerse photobioreactor.This developed photobioreactor is ideally to satisfy the cultivation requirements of terrestrial cyanobacteria. On the one hand, the fibre optics (glass rods), enclosed in the reactor, serves as colonizing surfaces for the organisms, and on the other hand the adherent growing organisms and their growth zones are optimally exposed to the supplied light. Moreover, the supplier of aerosol, integrated at the periphery of the reactor, provides water and nutrients enabling a nutrient-poor cultivation of the target microorganisms. The established geometry as well as the process control permit a cultivation which is highly adjusted to the production of valuables from terrestrial cyanobacteria. In this regard, the experimental set-up facilitates the induced production of specific valuables such as the pigment scytonemin by applying UV-A-radiation and desiccation as well as nitrogen depletion. Moreover, the optimization of exopolysaccharide production is forced, while its composition as well as its content will be modified by varying the supply of aerosol.