MRI/RUI: Instrumentation for Sequencing and Genomic Analysis

项目摘要在生物系的Peterson、Moyer和Young博士的监督下，已向西华盛顿大学授予一笔赠款，以支持购买DNA测序、基因分型和分析所需的核心仪器和软件。现在DNA测序很容易，再加上分子生物学和计算机科学的进步，使生物科学发生了革命性的变化。这项技术现在是许多学科基础研究的核心，也是未来研究人员和科学教育工作者培训不可或缺的一部分。根据这项提议建立的资金将促进11个不同实验室的初步研究，目前和即将获得资金。该仪器将支持广泛的领域，包括保护生物学、微生物多样性、进化生物学、植物功能基因组学、发育遗传学和蛋白质生物化学。最先进的仪器将通过允许我们使用现代技术进行严格的研究，进一步使赠款采购和续签成为可能。这一核心工具还将增强我们吸引和留住有能力的教授/研究人员的能力。除了建立和维护外部资助的研究项目外，我们还致力于通过在教师研究实验室指导本科生来整合研究和培训。培训将使我们的学生继续在地区和全国范围内获得认可，他们在生物技术行业工作，被研究生院和专业学校录取，并获得博士前学位。此外，我们正在修订我们的核心生物学课程，主要侧重于在所有班级整合分子生物学方法。总计，每年有500多名本科生将通过生物系的教学直接受益于这种仪器。通过WWU的综合实验室网络，文理学院和赫胥黎环境研究学院的附属项目以及伍德林教育学院未来的K-12科学教育工作者都可以使用这笔赠款支持的设备。总而言之，从教职员工和本科生到K-12理科学生的广泛人群都将受益。获得自动DNA测序和基因分型技术将加强学生教育和职业机会，同时促进基础科学研究。

Project SummaryA grant has been awarded to Western Washington University under the supervision of Drs. Peterson, Moyer and Young of the Biology Department to support the acquisition ofcore instrumentation and software required for DNA sequencing, genotyping,and analysis. The ease with which DNA can now be sequenced, coupled withadvances in molecular biology and computer science has revolutionized thebiological sciences. This technology is now central to basic research inmany disciplines, as well as integral to the training of future researchersand science educators.Funding from this proposal will facilitate primary research in elevendifferent labs with current and pending funding. A broad array of fieldswill be supported by this instrumentation, including Conservation Biology,Microbial Diversity, Evolutionary Biology, Plant Functional Genomics,Developmental Genetics, and Protein Biochemistry. State of the artinstrumentation will further enable grant procurement and renewal byallowing us to perform rigorous research using modern techniques. This coreinstrumentation will also enhance our ability to attract and retain capableprofessors/researchers.In addition to establishing and maintaining externally-funded researchprograms, we are dedicated to the integration of research and trainingthrough the mentoring of undergraduates in faculty research labs. Thistraining will allow our students to continue to be recognized regionallyand nationally with jobs in the biotechnology industry, admission tograduate and professional schools, and pre-doctoral awards. Furthermore,we are revising our core biology curriculum with a primary focus on theintegration of molecular biology approaches at all class levels. In all,over 500 undergraduate students per year will directly benefit from thisinstrumentation through instruction in the Biology Department. ThroughWWU's Integrated Laboratory Network the equipment supported by this grantwill be accessible to affiliated programs in the College of Arts andSciences and the Huxley College of Environmental Studies, as well as tofuture K-12 science educators enrolled in the Woodring College ofEducation. In all, a broad spectrum of people, ranging from faculty andundergraduates to K-12 science students will benefit. Access to automatedDNA sequencing and genotyping technologies will enhance student educationand professional opportunities while furthering fundamental scienceresearch.