SGER: Bio-doped Electronic Ceramics for Use in Microsensors

该项目涉及在溶胶-凝胶处理的过渡金属氧化物中加入酶或抗体，目的是生产用于检测有害化学和生物物种的先进生物传感器组件。建议的方法主要解决与材料相关的问题，以创建受控的多孔结构，其中生物分子可以很容易地“适合”和发挥作用。环境电子显微镜将被用来评估生物分子-溶胶-凝胶基质相互作用的性质。一个功能性生物传感器的原型将在项目结束时交付。该装置的工作原理是基于生物成分和分析物之间发生的生化反应导致气态氨的释放。该计划将开发的生物掺杂陶瓷有望在生物传感和医疗诊断领域以外的领域找到用途。检测有害气体物种和生物制剂的存在并监测其浓度的能力在人类生活和工作的任何环境中都是重要的。这项工作提出了一种新的方法，将陶瓷和生物材料混合在一起，以制造新型复合材料，用作微型生物传感器(如尿素检测器)中的传感元件。预计这些新材料将能够更快、更有效地检测污染物和危险物种。从事这个项目的学生将学习新材料加工和表征技术，并将获得生物传感器开发的宝贵技能，这将对他们的专业发展非常有用。本提案是针对尊敬的同事信：下一代化学和生物传感器和传感系统[NSF 02-112]提交的。

This project involves the incorporation of enzymes or antibodies in sol-gel processed transition metal oxides with the purpose of producing advanced biosensor components for the detection of harmful chemical and biological species. The proposed approach primarily addresses the material-related issues for creating controlled porous structures in which the biomolecules may easily "fit" and function. Environmental transmission electron microscopy will be employed to assess the nature of bio-molecule-sol-gel matrix interactions. A prototype of a functional biosensor will be delivered at the end of the program. The operating principle of this device is based on the release of gaseous ammonia as a result of biochemical reactions occurring between the biological component and the analyte. The bio-doped ceramics to be developed in this program are expected to find uses beyond the fields of bio-sensing and medical diagnostics.The ability to detect the presence and monitor the concentration of harmful gaseous species and biological agents is important in any setting where humans live and work. This work proposes a novel way to "mix" ceramic and biological materials together in order to produce novel composites for use as sensing elements in miniaturized and possibly implantable, biosensors (such as a urea detector). These novel materials are expected to allow for faster and more efficient detection of contaminants and dangerous species. The students working on this project will learn new materials processing and characterization techniques and will obtain valuable skills on biosensor development that will be very useful for their professional development.This proposal was submitted in response to the Dear Colleague Letter: Next Generation Chemical and Biological Sensors and Sensing Systems [nsf 02-112].