Protein Folding and Interaction Mechanics (A02)

在这个项目中，我们将使用光镊子进行单分子力学实验来研究蛋白质折叠和相互作用中涉及的力。总体而言，未来四年我们将努力实现三个主要目标。我们将解决的第一个问题涉及未折叠蛋白质链中结的定位。第二项研究将涉及力传感器的开发，这种传感器可以应用于活细胞中，以报告作用的机械力。最后一组实验将解决通过talin激活整合素所涉及的机械力的问题。

In this project, we will use single molecule mechanical experiments using optical tweezers to study forces involved in protein folding and interaction. Overall, we will pursue three major goals in the next four years. The first question we will address concerns the localisation of a knot in an unfolded protein chain. The second line of research will deal with the development of force sensors that can be applied in a living cell to report on the mechanical forces acting. The last block of experiments will address the question of the mechanical forces involved in the activation of integrin through talin.