Equipment Acquisition for the Clemson University Genomics Institute

在Jeffrey Tomkins博士的指导下，克莱姆森大学获得了一笔赠款，用于购买一台96毛细管DNA测序仪，用于BAC末端测序、鸟枪测序和EST测序;一台48毛细管测序仪，带有用于高信息含量BAC指纹分析（物理映射）的片段分析包;以及一台高分辨率飞行时间质谱仪，带有电喷雾电离样品引入接口。 仪器将被安置在克莱姆森大学基因组学研究所（CUGI），该研究所成立于1997年，负责基因组学的基础研究，向世界基因组学界分发BAC/EST资源，并为学生、博士后和其他访问科学家提供培训计划。这些小组是：1）BAC/EST资源中心，2）DNA测序，3）物理绘图，和4）生物信息学。 由于这笔赠款而获得的设备将提供给我们的DNA测序和物理绘图实验室，以便我们对基因组学社区的服务和培训计划能够有效地维持。此外，我们将通过获得一个关键的蛋白质分析工具，允许基础蛋白质组学研究和服务于社区，进一步扩大我们的研究，服务和培训功能。作为该补助金的一部分，我们将举办基因组学和蛋白质组学的夏季研讨会，这些研讨会将涉及定义明确的教学模块，并具有动手实验室经验。教育和推广将针对整个南卡罗来纳州的少数民族和较小的教学学院的教师，高中科学教师，以及整个州的少数民族本科生在他们的大三和大四之间的暑假。外联活动将利用新的设备升级，并通过克莱姆森大学的DNA学习中心进行协调。

A grant has been awarded to Clemson University under the direction of Dr. Jeffrey Tomkins for the acquisition of a 96-capillary DNA Sequencer for BAC end sequencing, shotgun sequencing, and EST sequencing; a 48-capillary sequencer with Fragment Analysis Package for High Information Content BAC Fingerprinting (physical mapping); and a high resolution, time of flight mass spectrometer with electrospray ionization sample introduction interface. The instrumentation will be housed at the Clemson University Genomics Institute (CUGI), which was established in 1997 to perform basic research in genomics, distribute BAC/EST resources to the world genomics community, and provide training programs for students, post-doctoral, and other visiting scientists.CUGI is structured to contain four basic working groups. These groups are: 1) BAC/EST Resource Center, 2) DNA Sequencing, 3) Physical Mapping, and 4) Bioinformatics. The equipment acquired as a result of this grant will provide to our DNA sequencing and physical mapping labs so that our service and training programs to the genomics community can be effectively maintained. Additionally, we will further expand our research, service and training functions by acquiring a key protein analysis tool permitting basic proteomics research and service to the community. As part of this grant, we will be hosting summer workshops in genomics and proteomics that will involve well-defined instructional modules with hands-on lab experience. Education and outreach will be targeted to faculty from minority and smaller teaching colleges throughout the state of South Carolina, High-School Science Teachers, and minority undergraduate students across the state in their summer break between their Junior and Senior year. The outreach will utilize the new equipment upgrades and be coordinated through the DNA Learning Center at Clemson University.