Increasing Breadth in the Separation Science Curriculum in Chemistry: Addition of HPLC and CE to Analytical, Organic, and Biochemistry Lab Instruction

化学(12)正在开发新的和改编的分离实验，以使我们的学生认识到高效液相色谱(HPLC)和毛细管电泳法(CE)的能力和局限性。该项目涉及将分离科学实验和概念纳入几个化学实验室，以便学生在反复接触的情况下，对影响分离的实验参数有更清楚的了解。学生们将根据约翰·沃尔特斯在一系列文章中所描述的小组形式进行合作(沃尔特斯，J.P.；分析。化学。1991年，63年，971a；分析。化学。1991、63、1077A；分析。化学。1991、63、1179A)。最终，学生们学会调整这些参数以改善分离。他们还通过分析具有不同目标的广泛样本来学习这些仪器技术的全面适用性，包括手性分析、方法开发和模拟以及蛋白质和多肽表征。化学专业的学生将会发现，分离科学对所有分子科学都具有明显的适用性，他们将变得更加适应能力，以比以前更完整和统一的方法解决化学问题。

Chemistry (12)New and adapted separation experiments are being developed to build awareness among our students of the capabilities and limitations of high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). The project involves incorporating separation science experiments and concepts across several chemistry laboratories so that, with repeated exposure, students have a clearer understanding of the experimental parameters that affect separation. Students will work together in an adaptation of the group format described by John Walters in a series of articles (Walters, J. P.; Analyt. Chem. 1991, 63, 971A; Analyt. Chem. 1991, 63, 1077A; Analyt. Chem. 1991, 63, 1179A). Ultimately students learn to adjust these parameters to improve separation. They also learn the full range of applicability of these instrumental techniques by analyzing a broad range of samples with different goals in mind, including chiral analysis, methods development and simulation, and protein and peptide characterization. Chemistry majors will discover that separation science has obvious applicability across all of the molecular sciences, and they will become more adaptable in their ability to solve chemical problems with a more complete and unified approach than before.