SBIR Phase I: New Encoding System for Detection of Pathogens

0339561这个小企业创新研究第一阶段项目需要开发一种新的芯片上电子编码珠阵列检测系统，用于同时多重检测病原体。这项技术将提供一种快速、高灵敏度的病原体或毒素鉴定方法。大多数用于快速检测病原体的当前系统是基于荧光染料标记和信号的光学检测，其易于光漂白并且在需要多重检测时可能达不到要求。 这种新的电子编码和基于芯片的系统具有高度的灵活性，可用于整个病原体、毒素或DNA/RNA的直接检测和鉴定，该方法将在许多生物分析领域产生广泛的影响，因为它将能够快速、高灵敏度和多重鉴定病原体或其毒素。拟议的研究将在分子诊断、传染病和生物制剂的检测方面产生广泛的影响和应用。 应用范围将从检测环境、农业和医学样本中的病原体和生物战剂到临床、分子诊断和法医应用中的传染病。

0339561This Small Business Innovation Research Phase I Project entails development of a novel on-chip electronic encoding bead-array detection system for simultaneous multiplexed detection of pathogenic agents. This technology will offer a quick and highly sensitive identification method for pathogens or toxins. Most current systems for the fast detection of pathogens are based on fluorescent dye labeling and optical detection of the signal, which are prone to photobleaching and are likely to fall short when multiplex detection is required. This new electronic encoding and chip-based system is highly flexibleand can be used for direct detection and identification of whole pathogens, toxins or DNA/RNA.The method will have a broad impact on a number of bioanalytical fields because it will be capable of rapid, highly sensitive and multiplexed identification of pathogens or their toxins. The proposed research will have broad impact and applications in molecular diagnostics, and detection of infectious disease and biological agents. The applications will range from the detection of pathogens and biological warfare agents in environmental, agricultural and medical samples to infectious disease in clinical, molecular diagnostic and forensic applications.