Equipment Acquisition for the OUHSC Laboratory for Genomics and Bioinformatics

在Allison Gillaspy博士的指导下，俄克拉荷马州大学健康科学中心（OUHSC）获得了一笔赠款，用于支持购买基因组解决方案G3S3000文库管理系统，该系统将用于管理来自各种来源的重组DNA，包括细菌和驯养或野生动植物。 高通量DNA测序及其相关技术对于生物学家的各种研究越来越有用，这些研究不仅包括基因组测序，还包括分子进化分析、微生物生态学和结构生物学等的高通量方法。 Genomic Solutions G3S3000库管理系统可以从存档的克隆库中复制和恢复单个克隆，并且非常灵活，这将增加每个研究项目的吞吐量，提高样本跟踪的准确性并减少技术人员的干预，从而降低纳税人支持的研究成本。 这台机器将位于OUHSC基因组学和生物信息学实验室，该实验室负责为俄克拉荷马州学术界提供各种分子生物学服务。 该设施为俄克拉荷马州的几个学术机构的至少92个用户提供服务，包括OUHSC、俄克拉荷马州大学、俄克拉荷马州州立大学和兰斯顿大学（俄克拉荷马州唯一的历史上的黑人学院或大学）。图书馆管理系统将有助于博士后研究员和研究生在实验室的92个学术用户，目前支持的研究相关的教育，并在OUHSC参加暑期实习的本科生。 因此，拟议的仪器将有显着的能力，提供这些服务的学术界在俄克拉荷马州的影响。 此外，两个正在进行的NSF资助的研究项目将大大受益于这种设备的可用性。这两个项目的重点是1）了解一种新的细菌寄生虫，2）鱼类进化。还有两个待决项目将需要拟议的设备，包括1）大量用作害虫生物防治剂的昆虫病原体的基因组测序和2）支持花生基因组测序倡议的DNA测序。 这项补助金的更广泛的意义在于能够为整个俄克拉荷马州的本科生和研究生以及博士后研究员提供现代基因组学技术的最先进教育。 这将丰富他们的教育经验，并促进国家内增强研究基础设施的发展。 该基金支持的研究将提高控制各种昆虫病原体的能力，并提高经济开发重要农业资源的能力。 反过来，这些将支持发展中的俄克拉荷马州生物技术产业，并为当地和州社区提供经济和教育利益。

A grant has been awarded to the Oklahoma University Health Sciences Center (OUHSC) under the direction of Dr. Allison Gillaspy for support of the purchase of a Genomic Solutions G3S3000 Library Management system, which will be used for the management of recombinant DNAs derived from a variety of sources, including bacteria, and domesticated or wild animals and plants. High-throughput DNA sequencing and its associated technologies are becoming useful for biologists for a variety of studies that not only include genome sequencing, but high-throughput methods for molecular evolution analysis, microbial ecology and structural biology, among others. The Genomic Solutions G3S3000 Library Management system can replicate and recover individual clones from archived clone banks, and is extremely flexible, which will increase throughput for each research project, increase the accuracy of sample tracking and reduce technician intervention, thereby reducing the costs of taxpayer-supported research. This machine will be located at the OUHSC Laboratory for Genomics and Bioinformatics, which is a facility responsible for providing a variety of molecular biological services to the Oklahoma academic community. The facility provides services to at least 92 users at several academic institutions in Oklahoma, including OUHSC, the University of Oklahoma, Oklahoma State University, and Langston University (Oklahoma's only Historically Black College or University). The Library Management system will contribute to the research-related education of postdoctoral fellows and graduate students in the laboratories of the 92 academic users that are currently supported, and undergraduate students that participate in summer internships at OUHSC. Thus, the proposed instrumentation will have significant impact on the ability to provide these services to the academic community in Oklahoma. In addition, two ongoing funded NSF research projects will benefit significantly from the availability of this equipment. These two projects are focused on 1) understanding a novel bacterial parasite, and 2) fish evolution. There also are two pending projects that will require the proposed equipment, including 1) the genome sequencing of an insect pathogen that is heavily used as a pest biocontrol agent and 2) DNA sequencing in support of a peanut genome sequencing initiative. The broader significance of this grant award is in the ability to provide state-of-the-art education in modern-day genomics techniques to undergraduate and graduate students, and postdoctoral research fellows throughout the state of Oklahoma. This will enrich their educational experience and promote the development of an enhanced research infrastructure within the state. Studies supported by this grant will enhance the ability to control a variety of insect pathogens, and enhance the ability to economically exploit an important agricultural resource. These, in turn, will support the developing Oklahoma biotechnology industry, and provide economic as well as educational benefit to the local and state community.