MRI: Acquisition of a Proteome Analyzer

在Jamil Momand博士的指导下，加州州立大学洛杉矶分校（加州州立大学洛杉矶分校）获得了一笔资助，用于部分支持蛋白质组分析仪。近年来，已经确定每个人可以表达25000种不同的基因组蛋白质。利用这些知识，科学界已经能够增加对这些基因的时间和表达水平如何影响细胞行为的理解，但在发育过程中基因表达的确切时间以及基因对环境毒素的反应尚不完全清楚。为了了解这些秘密，我们需要分析蛋白质组——生物体中表达的全部蛋白质。分离的蛋白质将被分割，碎片的质量将被测量。碎片质量的精确测量实际上是蛋白质的指纹。可通过互联网访问的公共数据库将用于根据指纹识别蛋白质。该项目的目标是增进对环境污染物、药物、自然发育、疾病和基因突变如何改变蛋白质表达和修饰的理解。蛋白质组分析仪是一种功能强大的工具，可以识别每种蛋白质并测量每种蛋白质行为的细微变化。蛋白质组分析仪将由七名研究人员与加州州立大学洛杉矶分校的本科生和硕士生一起进行研究。例如，莫曼德博士的实验室将识别出在高浓度过氧化氢环境下表达增加的蛋白质。Vellanoweth博士将鉴定与DNA元素结合的植物转录因子，并鉴定与花发育中抽苔过程相关的蛋白质。LaPolt博士的实验室将研究年轻周期性大鼠和中年无周期性持续发情的雌性大鼠脑垂体中蛋白质表达模式的差异。Krug博士的实验室将比较海洋软体动物Alderia modera发育过程中表达的蛋白质。蛋白质组分析仪将成为新的实验课程的一部分，该课程将训练本科生使用三种设备。在本课程中，学生将学习如何使用蛋白质组分析仪，新的600 MHz核磁共振和新的电子自旋共振机。加州州立大学洛杉矶分校是一所少数族裔服务机构，对少数族裔的教育和研究经历产生了重大影响。加州州立大学洛杉矶分校的毕业生中有很多是西班牙裔美国人。它在为本科生和研究生提供通往顶级博士课程的桥梁方面享有盛誉。收购蛋白质组分析仪将为其积极的教师提供一个研究工具，使他们能够进行前沿研究。因为蛋白质组分析仪将被纳入一个新的实验课程的课程，它将为所有加州州立大学洛杉矶分校的学生追求分子生命科学学位提供有价值的培训。

A grant has been awarded to California State University-Los Angeles (Cal State L.A.) under the direction of Dr. Jamil Momand for partial support of a Proteome Analyzer. In recent years it has been established that each human can express 25,000 different proteins from the genome. Using this knowledge, the scientific community has been able to increase its understanding of how the timing and expression levels of these genes affect the behavior of cells yet the exact timing of the expression of genes during development and the response of the genes to environmental toxins is not completely known. To understand these secrets one needs to analyze the proteome-the complete set of proteins expressed in an organism. Isolated proteins will be fragmented and the masses of the fragments will be measured. The precise measurement of the fragment masses is, in fact, a fingerprint of the protein. Public databases accessible through the internet will be used identify the protein based on the fingerprint. The goal of the project is to increase the understanding of how protein expression and modification is altered by environmental pollutants, drugs, natural development, diseases and genetic mutations. The Proteome Analyzer is a powerful tool that can identify each protein and measure subtle changes in each protein's behavior. The Proteome Analyzer will be used by seven investigators performing research with undergraduates and Masters Degree students at Cal State LA. For example, Dr. Momand's laboratory will identify proteins that increase expression in response to high levels of hydrogen peroxide. Dr. Vellanoweth will identify plant transcription factors that bind to DNA elements and identify proteins associated with the bolting process in flower development. Dr. LaPolt's laboratory will examine differences in patterns of protein expression in the pituitary glands of young cyclic versus middle-aged acyclic persistent-estrous female rats. Dr. Krug's laboratory will compare the proteins expressed during development in the marine mollusc Alderia modesta. The Proteome Analyzer will constitute part of a new laboratory course that will train undergraduates in the use of three pieces of equipment. In this course students will learn how to use the Proteome Analyzer, a new 600 MHz NMR, and a new Electron Spin Resonance machine.Cal State LA is a Minority-Serving Institution that has made a major impact on the educational and research experiences of minorities. Cal State LA graduates a very large number of students with Hispanic ancestry in the US. It has an excellent reputation in providing bridges to top-rated Ph.D. programs for its undergrad and graduate students. The acquisition of the Proteome Analyzer will give its motivated faculty a research tool to enable them to perform cutting-edge research. Because the Proteome Analyzer will be incorporated into the curriculum of a new laboratory course, it will provide valuable training to all Cal State LA students pursuing molecular life science degrees.