Connectivity and function of regenerated neurons in the cerebral cortex – from transplantation to direct neuronal reprogramming (A06)

在之前的资助期间，我们使用了选择性消融模型来显示从胎儿大脑皮层移植到成年小鼠视皮质的神经元的适当整合。在本项目中，我们的目标是首先将这一方法应用于更具临床相关性的模型，如老年小鼠的创伤性脑损伤，并检查移植神经元的髓鞘形成，这是一个关键问题，因为再生的成熟神经元无法形成髓鞘。在第二个项目中，我们将检验通过直接重编程从局部反应性神经胶质细胞获得的新神经元的整合和轴突髓鞘形成，基于我们在上一个资助期使用从COIL中读出的数据在创伤性脑损伤后实现的这一方法的突破。A08。

In the previous funding period we have used a selective ablation model to show the appropriate integration of neurons transplanted from fetal cerebral cortex into the adult murine visual cortex. In the present project we aim first to move this approach towards more clinically relevant models such as traumatic brain injury also in older mice and examine myelination of the transplanted neurons, a key question as regenerating mature neurons fail to be myelinated. In the second project part we will examine the integration and axonal myelination of new neurons obtained by direct reprogramming from local reactive glial cells, based on the breakthrough in this approach after traumatic brain injury we achieved in the last funding period using the read-outs from in coll. with A08.