RNA-protein-protein interactions of plant RNA editing factors

植物线粒体和质体中的RNA编辑过程需要几种蛋白质组分才能有效地将靶RNA中的特定C核苷酸转变为U。PPR蛋白是在单个或几个编辑位点附近识别独特RNA序列基序的因子。每一种MORF蛋白都影响许多位点，但它们的确切功能仍然是谜。本项目旨在分析目标rna、PPR和MORF蛋白之间的rna -蛋白和蛋白-蛋白相互作用，以更好地定义它们各自的功能和它们在编辑体中的合作。此外，蛋白质-蛋白质相互作用搜索将用于寻找拟南芥编辑体的其他成分，包括仍未知的酶活性。

The RNA editing process in mitochondria and plastids of plants requires several protein components for efficient alteration of specific C nucleotides to U in target RNAs. PPR proteins are identifying factors for a unique RNA sequence motif near single or few editing sites. Each of the MORF proteins affect numerous sites, but their precise function is still enigmatic. This project aims to analyze RNA-protein and protein-protein interactions between the target RNAs, the PPR and the MORF proteins to better define their respective function and their cooperation in the editosome. Furthermore, protein-protein interactive searches will be employed to search for additional components of the editosome in Arabidopsis thaliana including the still unknown enzymatic activity.