Nanotox: Biochemical, Molecular and Cellular Responses of Zebrafish Exposed to Metallic Nanoparticles

[05:40920] Barber该提案涉及金属纳米颗粒对斑马鱼的毒性作用的生物学、组织学和分子研究。这些鱼将暴露在不同浓度的铝、银、镍和二氧化钛纳米颗粒的水悬浮液中96小时。毒性效应将在鳃、肝、肠和肾组织的形态学、生化和分子水平上进行评估。主要目的之一是比较金属在溶解状态和纳米颗粒状态以及不同大小和形状的纳米颗粒的毒性。假设是金属纳米颗粒会产生不同于水溶液中的金属的毒性作用。该提案将利用新到达的斑马鱼微阵列来表征不同处理水平下的基因表达，然后对15个最突出的基因进行定量PCR。还将测试一种体外模型系统，作为水生物种中金属纳米颗粒毒性测定的替代方法。

0540920 Barber The proposal is concerned with biological, histological and molecular studies of toxic effects of metal nanoparticles on zebrafish. The fish will be exposed for 96 hours to aqueous suspensions of aluminum, silver, nickel, and titania nanoparticles of various concentrations. Toxicity effects will be evaluated at the morphological, biochemical and molecular levels in gills, liver, intestine, and kidney tissues. One of the main objectives is to compare toxicity of metals in the dissolved and nanoparticle state as well as the nanoparticles of different sizes and shape. The hypothesis is that the metal nanoparticles will produce toxic effects that are different from that of metals in aqueous solution. The proposal will take advantage of newly arriving zebrafish microarrays to characterize gene expression at different treatment levels, followed by quantitative PCR of the 15 most prominent genes. An in vitro model system will also be tested as an alternative for a toxicity assay of metal nanoparticles in aquatic species.