Systemic acquired resistance in plants - an omics approach to pathogen defence signalling

植物的免疫在一定程度上是基于相应的植物抗性(R)基因产物对病原体效应分子的识别。这种类型的免疫伴随着在植物剩余的健康组织中诱导一种持久的广谱防御，即系统获得性抗性(SAR)。合成孔径雷达以低能源成本维持状态，合成孔径雷达信号组件可以用来保护作物免受疾病的侵袭，而不会造成产量的显著损失。因此，我们的目标是确定影响移动合成孔径雷达信号产生和/或传输的合成孔径雷达信号组件。为此，我们将使用模式植物拟南芥和突变体eds1，它不能产生或传递SAR信号来响应细菌效应器AvrRpm1。首先，将用不同的基于质谱学的技术分析表达AvrRpm1的野生型(Wt)和eds1植物的提取物的肽、脂和极性小分子含量。其次，将在拟南芥和包括作物番茄在内的其他植物物种中检测由此确定的候选SAR信号化合物的防御诱导潜力。最后，我们将通过转录谱研究新的SAR信号如何影响其他调控过程，例如那些影响干旱胁迫耐受性的过程。

Immunity in plants is based in part on recognition of pathogen effector molecules by corresponding plant RESISTANCE (R) gene products. This type of immunity is accompanied by the induction of a long-lasting broad spectrum defence, systemic acquired resistance (SAR), in the remaining healthy tissues of the plant. The SAR state is maintained at low energy cost, and SAR signalling components could be used to protect crops from disease without significant loss of yield. Therefore, we aim to identify SAR signalling components that affect the generation and/or transmission of mobile SAR signals. To this end, we will use the model plant Arabidopsis thaliana and a mutant, eds1, which is incapable of generating or transmitting SAR signals in response to the bacterial effector AvrRpm1. First, the peptide, lipid, and polar small molecule content of extracts of AvrRpm1-expressing wild type (wt) as compared to eds1 plants will be analyzed by different mass spectrometry-based techniques. Second, the defence-inducing potential of thus identified candidate SAR signalling compounds will be examined in A. thaliana and other plant species, including the crop plant tomato. Finally, we will study how new SAR signals affect other regulatory processes, e.g. those affecting drought stress tolerance, by transcript profiling.