NER: Protein chips of attoliter protein droplets on microelectrodes

该项目将合成具有不同长度和组分之间比例的量身定制的嵌段共聚物，并测试几种感兴趣蛋白质的液滴生成。聚合物产生浓缩的蛋白质相，然后交联并定位到电极上。溶剂可以被除去，然后进行预浓缩。作者展示了电极上的精确定位，相当于信号放大。这些成分将共同形成新型的二维蛋白质微芯片。计划中的工作需要合成一种聚合物，无论溶液中存在什么蛋白质，它都能分相分离，并在形成的液滴中捕获足够数量的每种蛋白质。第二个具体目标是开发微电极上液滴局部生成和沉积的程序。

0609387VekilovThis project will synthesize tailored block copolymers with varying length and ratios between components, test droplet generation with several proteins of interest. The polymer creates concentrated protein phases and then is cross-linked and localized onto electrodes. The solvent can be removed and hence a pre-concentration follows. The authors show precise localization on electrodes, amounting to signal amplification. Together, the components will form novel 2D protein microchips. The planned work requires the synthesis of a polymer that will phase separate regardless of the proteins present in the solution and entrap sufficient quantities of each protein in the formed droplets. The second specific aim is to develope procedures for localized generation and deposition of the droplets over microelectrodes.