International Research Fellowship Program: Effects of Climate Change on a Marine Invertebrate

0700551 Fangue国际研究奖学金计划使美国科学家和工程师能够在国外进行9到24个月的研究。 该计划的奖项提供了联合研究的机会，以及使用独特或互补的设施，专业知识和国外的实验条件。Fangue将与墨西哥恩塞纳达下加利福尼亚自治大学海洋研究所（IIO-UABC）的Eugenio Carpizo-Ituarte博士和Gretchen E.圣巴巴拉的加州大学海洋科学研究所。 该项目由国际科学和工程办公室美洲方案提供支助，其中心目的是研究海洋无脊椎动物对气候变化的生理反应。 在未来的几十年里，科学家们预计会有越来越多的物种入侵和物种分布范围的变化，以应对全球气候变化。 确定在气候变化所带来的新环境中设定物种范围界限和影响生理表现的过程和机制至关重要。紫海胆（Strongylocentrotus purpuratus）的分布范围很广，从不列颠哥伦比亚省的温哥华岛到墨西哥的蓬塔尤金尼亚。此外，海胆的幼虫扩散和定居阶段受到全球变暖情景中预测变化的因素的严重影响。我将研究海胆胚胎和幼虫对温度和酸化压力的反应，以及这些压力在生态相关水平上的组合（基于当前的气候变化预测）。 重点将放在最南部的人口（蓬塔Eugenia）的物种范围，因为全球变化的直接后果之一是一个可能的转移极物种分布，或“热点”的物种损失，由于热应力。 此外，多个海胆种群从其分布范围也将从地理格局的角度进行调查。 使用从生理性能和生存措施，以及转录组分析与cDNA微阵列和定量PCR获得的结果，我希望作出预测的潜在影响气候变化对幼海胆的生理性能。 此外，通过识别不同的基因表达模式（转录丰度和物种），这可能是重要的海胆的温度和酸化压力的反应，PI将有助于推进有关生态相关基因在气候变化的背景下的假设。 此外，协同应激源的使用将产生一个重要的数据集，确定海洋无脊椎动物对温度和酸化的生理反应。

0700551FangueThe International Research Fellowship Program enables U.S. scientists and engineers to conduct nine to twenty-four months of research abroad. The program's awards provide opportunities for joint research, and the use of unique or complementary facilities, expertise and experimental conditions abroad.This award will support a twenty-four-month research fellowship by Dr. Nann A. Fangue to work with Dr. Eugenio Carpizo-Ituarte of the Instituto de Investigciones Oceanologicas of the Universidad Autonoma de Baja California (IIO-UABC), Ensenada Mexico and with Dr. Gretchen E. Hofmann, Marine Science Institute of the University of California at Santa Barbara. Support for this project is provided by the Office of International Science and Engineering's Americas Program.The central aim of this project is to study the physiological performance of a marine invertebrate in response to climate change. In the coming decades, scientists expect increasing numbers of species invasions and shifts in species range distributions in response to global climate change. It is critical to determine the processes and mechanisms that set species range boundaries and influence physiological performance in novel environments presented by climate change. The purple sea urchin, Strongylocentrotus purpuratus, has a broad distribution range from Vancouver Island, British Columbia to Punta Eugenia, Mexico. As well, sea urchins have larval dispersal and settlement phases that are heavily influenced by factors predicted to change in global warming scenarios. I will investigate the response of sea urchin embryos and larvae to temperature and acidification stressors, as well as these stressors in combination at ecologically relevant levels (based on current climate change predictions). Emphasis will be placed on the southern-most population (Punta Eugenia) of the species range because one of the direct consequences of global change is a possible shift poleward in species distributions, or "hot spots" of species loss, as a result of thermal stress. In addition, multiple sea urchin populations from across their distribution range will also be investigated from a biogeographical patterning perspective. Using results obtained from physiological performance and survival measures as well as transcriptome profiling with cDNA microarrays and quantitative PCR, I hope to make predictions about the potential impacts of climate change on the physiological performance of larval sea urchins. In addition, by identifying distinct patterns of gene expression (transcript abundance and species) that may be important in the sea urchin's response to temperature and acidification stressors, the PI will help to advance hypotheses about ecologically relevant genes in the context of climate change. Furthermore, the use of synergistic stressors will generate an important data set identifying physiological responses to temperature and acidification in a marine invertebrate.