NER: Actin Filament Crosslinking in vitro and in vivo using Purified and Isolated Carbon Nanotubes ? A Potential Cancer Therapy

0708418 Dahl这项拟议的研究将建立亚细胞重组与将各向异性分子掺入细胞的毒理学效应之间的关系。鉴于碳纳米管毒性的相互矛盾的证据，PIS将仔细研究表征良好和纯化的碳纳米管与活细胞的相互作用。碳纳米管与细胞的相互作用将从进入细胞的点起受到光谱和显微镜的密切监测。这一结果将启动用于细胞治疗的新型纳米材料的开发，并加深对细胞骨架重组细胞功能的理解。在分析和了解了碳纳米管对细胞行为的影响后，研究人员将对碳纳米管的大表面积进行修饰，以设计和开发非破坏性的靶向癌症治疗方法。

0708418 Dahl This proposed research will establish a relationship between subcellular reorganization and toxicological effects to incorporation of anisotropic molecules into cells. in light of conflicting evidence of the CNT toxicity, the PIs will undertake careful study of the interaction of well-characterized and purified CNTs with live cells. The CNT-cell interactions will be closely monitored spectroscopically and microscopically from the point of entry into the cell. The results will launch the development of novel nanomaterials for cellular therapies as well as deepen the understanding of cytoskeletal reorganization cellular functions. After analyzing and understanding the effects of CNTs on cell behavior, the investigators will modify the large surface areas of CNTs to design and develop non-destructive targeted cancer therapies.