Optische Pinzette mit Doppelfalle sowie Teilchennachverfolgung

“与我们已建立的具有视频检测功能的定制单光束光镊装置相比，具有双焦点四象限二极管检测功能的商用双光束光镊装置将具有几个重要的概念优势。最突出的好处是在大约10 μs的高得多的时间分辨率中被发现。这将使我们能够以比现有设备更精确和详细的方式研究单个生化对相互作用事件，如受体-配体-相互作用。此外，一个显著增加的时间粒子跟踪分辨率将为我们提供继续和推进我们在随机热力学的新颖和有前途的分支实验研究的能力。此外，具有光学陷阱的双光束配置可以在空间和强度上自由操纵，在任何类型的双胶体实验中都可以增加对抗漂移效应的时间稳定性。因此，与我们目前的单束移液器方法相比，这种配置相当优越，并将导致改进的结果。结合技术上要求的平面设计，这些可自由操纵的陷阱可以相对容易地实现自动搜索和陷阱算法。这种自动捕获算法将是朝着相关实验完全自动化迈出的重要一步，最终使我们能够以相当高的吞吐量和统计量测量单个生化和生物物理事件。”

„Compared to our established custom made single beam optical tweezers setup with video detection, a commercial dual beam optical tweezers device with bifocal four-quadrant-diode detection, will have several significant conceptual advantages. The most outstanding benefit is to be found in the much higher temporal resolution of approximately 10 μs. This would enable us to study single biochemical pair interaction events, like receptor-ligand-interaction, in a much more precise and detailed fashion than it is feasible with the present equipment. Moreover, a significantly increased temporal particle tracking resolution would provide us with the capability to continue and advance our experimental research on the novel and promising branch of stochastic thermo-dynamics. Furthermore, a dual beam configuration with optical traps being freely steerable in space and strength offers an increased temporal stability against drift effects during any kind of two-colloid experiments. As a result, this configuration is considerably superior compared to our present single beam pipette approach and will lead to improved results. In combination with the technically required flat design of the used fluidic cells, these freely steerable traps allow a comparably easy implementation of an automated seek-and-trap algorithm. Such an auto-trapping algorithm would be a major step forward towards a complete automation of the pertinent experiments which would eventually enable us to measure single biochemical and biophysical events with reasonably high throughput and statistics.“