SGER: Evaluating the influence of nanoparticles on soil microbial diversity.

SGER关于资助Vishal Shah Dowling Colleed的提案编号：0714685 SGER：评估纳米颗粒对土壤微生物多样性的影响。摘要通过该提案的资金支持，将开发一种可以扩大到田间水平的技术。这项技术将是无污染的，不需要寄生能量输入。目前，美国联邦或州的指导方针没有明确规定纳米粒子的生产者在将其引入环境之前进行毒理学研究(关于人类和环境)的任何义务。这些纳米颗粒通过供人类食用或意外释放的产品在环境中找到了存在的方式。例如，纳米颗粒向土壤中的释放是一系列活动的结果，包括通过土壤和水修复技术故意释放、潜在的农业用途(例如化肥)以及可能通过空气、水和应用于土地的污水污泥的非故意释放。因此，尽管纳米颗粒已经找到了进入环境的途径，但人们对纳米颗粒对环境的影响知之甚少。通过目前的方案，我们建议调查游离纳米颗粒对土壤微生物群落的影响，并利用多种测量工具和开展微观研究来评估土壤健康。将选择五种不同的纳米颗粒，利用两种土壤类型来研究对土壤微生物多样性的生态毒理效应。将通过活力计数、脂肪酸甲酯分析、Biolog生态平板、植物毒性、土壤物理化学分析和酶研究进行评估。

SGER Proposal for FundingVishal ShahDowling CollegeProposal No: 0714685SGER: Evaluating the influence of nanoparticles on soil microbial diversity.AbstractThrough the funding of this proposal, a technology that can be scaled up to field levels will be developed. The technology will be non-polluting and would require no parasitic energy input.Currently, federal or state guideline in United States does not specify any obligation on the producers of nanoparticles to carry out toxicological studies (on human and environment) before it is introduced into the environment. These nanoparticles find there way in the environment through products for human consumption or accidental release. For example, release of nanoparticles into soil is a result of number of activities, including deliberate release via soil and water remediation technologies, potential agricultural uses (e.g. fertilisers) and potential unintentional releases via air, water and from sewage sludge applied to land. Thus, while nanoparticles have found its way into the environment, little knowledge exists on the influence of nanoparticles on environment. Through the current proposal we propose to investigate the influence of free nanoparticles on microbial community in soil and evaluate soil health using multiple measurement tools and carrying out studies in microcosms. Five different nanoparticles will be selected to investigate the eco-toxicological effect on soil microbial diversity using two soil types. Evaluation will be carried out through viability count, Fatty Acid Methyl Ester analysis, Biolog Eco plates, phyto-toxicity, physico-chemical analysis of soil and enzymatic studies.