SGER: Can specific proteins be detected and quantified in soil using proteomics techniques?

SGER提案CBET 0738040蒂姆·马特斯爱荷华大学能否使用蛋白质组学技术检测和定量土壤中的特定蛋白质？本研究的主要目的是开发和应用蛋白质组学技术来检测和定量特定的蛋白质？来自类诺卡氏菌菌株的烯烃单加氧酶（AkMO）的α亚基（EtnC），其具有降解氯乙烯（VC）的能力。AkMO酶催化VC好氧生物降解的第一步，VC是氯化溶剂三氯乙烯生物降解的有毒中间体，三氯乙烯是一种广泛分布的污染物，是土壤和地下水。在复杂的土壤系统中检测AkMO（通过亚基EtnC）的能力将是有用的生物标志物，以信号的VC同化细菌在土壤中的存在。这个建议符合SGER的标准，因为它是一个创新的想法，并满足高风险高回报的标准。PI是一个年轻的终身助理教授，谁是很有资格进行拟议的工作，并有？最先进的分析仪器（液相色谱法与串联质谱法[lc-ms-ms]），用于测量蛋白质大分子。氯化溶剂对土壤和地下水的污染是一个重大的社会问题。这项工作有可能开辟一个新的研究领域，使用蛋白质组学技术的生物标志物的发展。

SGER ProposalCBET 0738040Tim MattesUniversity of IowaCan specific proteins be detected and quantified in soil using proteomics techniques?The primary objective of this research is to develop and apply proteomics techniques to detect and quantify a specific protein?the alpha subunit (EtnC) of the enzyme alkene monooxygenase (AkMO) from a strain of the bacterium Nocardioides, which has the capability of degrading vinyl chloride (VC). The AkMO enzyme catalyzes the first step in aerobic biodegradation of VC, which is a toxic intermediate in the biodegradation of chlorinated solvent trichloroethene, a widely distributed contaminant is soils and ground water. The ability to detect AkMO (via the subunit EtnC) in complex soil systems would be useful as a biomarker to signal the presence of VC-assimilating bacteria in soils. This proposal fits the criteria for an SGER in that it is an innovative idea and satisfies the high risk-high reward criterion. PI is a young tenure-track assistant professor, who is well qualified to conduct the proposed work and has ?state-of-the-art? analytical instrumentation at his disposal (liquid chromatography coupled with tandem mass spectrometry[lc-ms-ms]) for measuring protein macromolecules. Soil and ground-water contamination by chlorinated solvents is a significant societal problem. This work has the potential of opening a new area of research using proteomics techniques for the development of biomarkers.