High Resolution Magnetophoresis using Multilayered Magnetic Nanodisks

ECCS-0901849：摘要本研究的目的是证明利用多层磁性纳米盘作为标记物可以实现高分辨率的磁多路生物分离。这种新的磁泳方法允许根据其独特的磁特性连续分离亚100 nm尺寸的磁性纳米盘。智力优势：所提出的多层纳米盘由两个磁性层组成，由两个覆盖层的非磁性层隔开，可以提供宽范围的磁特性可调性，这导致了两个重要的优势，优于常用的超顺磁性氧化铁纳米颗粒：开辟了磁多路复用的新可能性，并填补了从30 nm到200 nm的超顺磁性纳米颗粒尺寸间隙，以提供用于生物分离的高磁矩磁性标记。在微流体布置中，这些纳米盘将经受以三角波格式随时间变化的磁场。具有不同饱和场的纳米盘可以连续分离。由于标记物的磁性和磁场是已知的，因此整个分离过程可以很容易地建模，这将为我们提供一个方便的工具来优化不同类型分离的条件。更广泛的影响：分离是任何生化分析的重要组成部分。所提出的分离方法模拟了广泛使用的电泳，但用磁场代替电场。磁场不干扰生物过程和材料，并且可以在磁体与任何液体之间没有物理接触的情况下外部施加。磁力与离子强度、pH值或表面电荷无关。可以看出，如果成功的话，这种方法将为生物分离提供重要的替代方案。此外，它可以将有价值的多路复用引入许多高灵敏度的生物传感检测方法中。这两种方法都将在许多生物学和生物医学研究领域产生广泛的影响。此外，该项目提供了一个出色的场所，将研究活动与纳米生物技术的大学生和研究生的教育和培训相结合，纳米生物技术被认为是美国在全球经济竞争和国家安全中最具潜在价值的技术之一。在纳米生物技术本科教学中，将建立三个实验模块，探索一种新的实习方法;实验导向的即时教学此外，通过UT阿灵顿现有的三个夏令营，数百名K-12学生将接触到这项研究。其中，大约30%是西班牙裔学生。学生们肯定会受到积极的影响，并可能激发他们对科学和工程的兴趣。

ECCS-0901849: High Resolution Magnetophoresis Using Multilayered Magnetic NanodisksAbstractThe objective of this research is to demonstrate that a high resolution magnetic multiplexed bioseparation can be achieved by using multilayered magnetic nanodisks as labels. This new magnetophoretic approach allows a continuous separation of sub-100 nm sized magnetic nanodisks according to their distinct magnetic properties.Intellectual Merit: The proposed multilayered nanodisks, consisting of two magnetic layers separated by a non-magnetic layer with two capping layers, can provide a wide-range tunability of magnetic properties, which results in two important advantages over commonly used superparamagnetic iron oxide nanoparticles: opening up the new possibility of magnetic multiplexing and filling the superparamagnetic nanoparticle size gap, from 30 nm to 200 nm, to provide high-moment magnetic labels for bioseparation. In a microfluidic arrangement, these nanodisks will be subjected to a magnetic field varying with time in a triangle wave format. Nanodisks with different saturation fields can be continuously separated. Since magnetic properties of labels and magnetic fields are exactly known, the whole separation process can be easily modeled, which will provide us a convenient tool to optimize conditions for different kind of separations. Broader Impacts: Separation is an important part of any biochemical analysis. The proposed separation method mimics widely-used electrophoresis, but replacing electric field with magnetic field. Magnetic field does not interfere with biological process and materials, and can be applied externally without physical contact between magnet and any liquid. Magnetic forces are independent on ionic strength, pH or surface charges. It can be seen that this proposed approach, if successful, will provide an important alternative for bioseparation. Moreover, it can introduce valuable multiplexing into many highly sensitive detection methods for biosensing. Both of these will have a broad impact in many biological and biomedical research fields. Also, this project offers an outstanding venue to integrate research activities with the education and training for college and graduate students in nanobiotechnology, which is considered as one of the most potentially valuable technologies for U.S. in the global economic competition and for national security. Three experiment modules will be created for a new practicum approach on undergraduate nanobiotechnology teaching; ?experiment-oriented just-in-time teaching?. In addition, through three existing summer camps at UT Arlington, several hundreds of K-12 students will be exposed to this research. Among them, about thirty percent are Hispanic students. The students will certainly be affected positively and may spark their interest in science and engineering.