High-speed three dimensional coherent nonlinear imaging with temporal-focus depth scanned holography

“这项奖励是根据2009年美国复苏和再投资法案（公法111-5）资助的。”相干非线性激光显微镜（CNLM）如三次谐波（THG）和二次谐波（SHG）世代显微镜是广泛应用的成像工具。然而，到目前为止，它们的应用已经避开了需要快速图像采集的系统。我们提出了一种利用数字全息技术与驱动超快激光脉冲的快速时空扫描相结合的方法来快速获取非线性显微镜形成的三维图像。智力优势：本研究将发展这些能力，可用于研究许多过程的快速动力学。利用时空聚焦的最新进展，将有可能开发出具有相干非线性对比机制的超高速三维成像的新形式。我们将建立对三维非线性成像能力的理解，并将更好地理解如何优化横向和纵向（轴向）分辨率，同时获得关于物体的3D信息。更广泛的影响：本研究将主要集中在THG显微镜；但该技术也适用于其他相干非线性显微镜，如二次谐波产生（SHG）和相干反斯托克斯拉曼光谱（CARS）。增加的采集率可能与全息显微镜将允许微观到毫秒时间尺度动力学的研究。本科研究人员将参与该项目的各个方面，重点是超快和非线性光学以及显微镜的研究领域。研究成果将被纳入PI教授的课程，特别是超快光学和非线性光学。此外，PI将继续修改并为k -2年级学生提供“光学秀”。

"This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5)."Coherent nonlinear laser microscopy (CNLM) such as third harmonic (THG) and second harmonic (SHG) generation microscopes are widely used imaging tools. However, their application to date has eluded systems requiring rapid image acquisition. We propose to develop a method for rapidly acquiring three-dimensional (3D) images formed by nonlinear microscopy by means of digital holography coupled with rapid spatio-temporal scanning of the driving ultrafast laser pulses. Intellectual Merits: The proposed research will develop these capabilities which can be used to study fast dynamics of many processes. Making use of recent advances in spatio-temporal focusing, it will be possible to develop new forms of very high speed 3D imaging with coherent nonlinear contrast mechanisms. We will build an understanding of the capabilities of 3D nonlinear imaging and will better understand how to optimize both transverse and longitudinal (axial) resolution, while obtaining 3D information about an object.Broader Impacts: This research will primarily focus on THG microscopy; but the technique is applicable to other coherent nonlinear microscopes, such as second-harmonic generation (SHG) and coherent anti-Stokes Raman spectroscopy (CARS). Increased acquisition rates made possible with holographic microscopy will permit the study of micro- to millisecond-timescale dynamics. Undergraduate researchers will be involved in all aspects of this project focusing on research areas of ultrafast and nonlinear optics, and microscopy. Research findings will be incorporated into courses taught by the PI, particularly Ultrafast Optics and Nonlinear Optics. In addition, the PI will continue revising and delivering his "optics show" for K-2nd graders.