The protein sorting and assembly maschineries of the mitochondrial outer membrane (P22)

线粒体外膜蛋白的分选组装机制(SAM)和线粒体输入复合体(MIM)参与了线粒体外膜蛋白的生物发生。我们发现，这两个蛋白质分选机制形成了一个动态网络，以介导不同类型的膜整合蛋白质的生物发生。在这个项目中，我们将测试我们的假设，即偶联到不同的伙伴蛋白质调节两个蛋白质机械的底物特异性。因此，我们将确定伙伴蛋白和前体蛋白的对接位置，并分析与伙伴蛋白的相互作用在蛋白质分选中的作用。

The sorting and assembly machinery (SAM) and the mitochondrial import (MIM) complex mediate the biogenesis of mitochondrial outer membrane proteins. We have discovered that both protein sorting machineries form a dynamic network to mediate biogenesis of different types of membrane-integrated proteins. In this project we will test our hypothesis that coupling to different partner proteins modulates the substrate specificity of both protein machineries. Therefore, we will define docking sites for partner proteins and precursor proteins and analyze the function of the interaction with partner proteins in protein sorting.