Conformational dynamics of signal transducers in photo- und chemotaxis (P10)

Project P10 （Steinhoff）通过聚集在质膜微室中的嗜盐古菌Natronomonas pharaonis的光感受器/换能器NpSRII/NpHtrII，剖析了细菌趋化和光性的信号转导机制。利用EPR光谱，该小组将(a)通过比较甲基化和去甲基化状态来剖析信号复合物内的适应和动态变构机制，(b)分析通道视紫质泵送Ca2+离子的机制，以了解光诱导相关系统的构象变化。

Project P10 (Steinhoff) dissects the mechanism of signal transduction in bacterial chemo- and phototaxis via the photoreceptor/transducer NpSRII/NpHtrII of the halophilic archaeon Natronomonas pharaonis, which are clustered in microcompartments of the plasma mem-brane. Taking advantage of EPR spectroscopy, the group will (a) dissect the mechanism of adaptation and dynamic allostery within the signaling complex by comparing the methylated and demethylated states, and (b) analyze the mechanism of Ca2+-ions pumping by channelrhodopsin to understand light induced conformational changes in a related system.