Role of the presynaptic protein Mover at auditory brain stem synapses (B02)

动子是最近发现的突触前机制的组成部分。我们计划i）确定Mover在耳蜗核急性切片中的兴奋性和抑制性突触中的作用，ii）表征其对听力受损小鼠体内稳态可塑性的贡献，以及iii）通过分析Mover在大脑和培养的神经元中的表达和定位如何被调节来确定潜在的分子机制。我们的主要工具将是我们在第一节课中创建的floxed Mover鼠标线。使用Cre线，我们将删除Mover在不同的神经元子集，研究突触传递的膜片钳和突触结构的光和电子显微镜。

Mover is a recently identified component of the presynaptic machinery. We plan to i) determine the role of Mover at excitatory and inhibitory synapses in acute slices of the cochlear nucleus, ii) characterize its contribution to homeostatic plasticity in hearing impaired mice, and iii) determine the underlying molecular mechanisms by analyzing how the expression and localization of Mover are regulated in the brain and in cultured neurons. Our primary tool will be a floxed Mover mouse line that we created in the first period. Using Cre lines we will delete Mover in distinct subsets of neurons, to study synaptic transmission by patch clamping and synaptic architecture by light and electron microscopy.