MRI: Acquisition of a Transmission Electron Microscope for the Core, Multi-User Microscopy Facility at Appalachian State University

阿巴拉契亚州立大学（ASU）艺术与科学学院的德维尔显微镜设施（DMF）正在使用一种新的透射电子显微镜（TEM）来改善和加强ASU和北卡罗莱纳州西部的教职员工和学生的研究和教学。透射电镜是研究动植物组织内部细胞和超微结构以及微生物的重要工具。它对许多其他科学学科的教学和研究也非常重要，包括物理、化学、地质和材料科学。获得的仪器是JEOL JEM-1400 TEM。DMF是所有阿巴拉契亚州立大学院系以及社区学院、高中和北卡罗莱纳州西部阿巴拉契亚地区的工业的核心多用户显微镜设备。该仪器对研究人员产生重大影响的一些直接应用包括研究微管在根发育和形态发生中的作用；强光胁迫下植物干细胞产生活性氧的定位研究小鼠颈椎重塑过程中微血管改变的表征鱼类鳃中蛋白质的超微结构定位以及用于化学传感的光纤端面金属纳米结构研究。此外，这一新的TEM将显著提高阿巴拉契亚州立大学生物和物理显微镜课程的教学质量。阿巴拉契亚州立大学是一所发展迅速的公立大学，位于北卡罗来纳州西部，许多学生都是第一代大学生。因此，应用这种新仪器的主要目标之一是培养许多跨生物、物理、化学、地质和其他学科的积极的本科生。新的特教小组还将在目前的几个推广项目中发挥重要作用，包括阿巴拉契亚学生体验教育学院（AppalSEED），这是一个为期一年的项目，重点培训来自北卡罗莱纳州西部妇女和非洲裔美国学生等代表性不足群体的在家接受教育的学生和高中生。最后，新的TEM将增强DMF对周边社区的服务能力，并进一步加强生物学家、物理学家和其他科学家以及北卡罗莱纳州西部其他大学的教职员工和学生之间的合作。公众可在DMF网站（http://www.casmifa.appstate.edu/index.html）的专门页面上查看项目成果。

The Dewel Microscopy Facility (DMF) in the College of Arts & Sciences at Appalachian State University (ASU) is using a new transmission electron microscope (TEM) to improve and enhance faculty, staff, and student research and teaching at ASU and in western North Carolina. A TEM is an essential tool for studying internal cellular and ultra-structures of animal and plant tissues, as well as microorganisms. It also is very important for teaching and research in many other scientific disciplines, including physics, chemistry, geology and materials science. The acquired instrument is the JEOL JEM-1400 TEM. The DMF is a core, multi-user microscopy facility for all Appalachian State University departments as well as community colleges, high schools and industry in the Appalachian region of western North Carolina. Some of the immediate applications where this instrumentation has significant impact for researchers include investigation of the roles of microtubules in root development and morphogenesis; localization of plant stem cells producing reactive oxygen species under high light stress; characterization of microvascular alterations during cervical remodeling in mice; ultrastructural localization of proteins in the gills of fishes; and studies of metallic nanostructures on end-faces of optical fibers for chemical sensing applications. In addition, this new TEM will significantly improve the teaching quality of biology and physics microscopy courses at Appalachian State University.Appalachian State University is a fast growing public university located in western North Carolina, many of whose students are first generation college attendees. Therefore, one of the main goals in the application of this new instrumentation is to train many motivated undergraduate students across biology, physics, chemistry, geology, and other disciplines. The new TEM will also play a vital role in several current outreach programs, including the Appalachian Student Experience-based Education (AppalSEED) Academy, which is a year-long program and focuses on training home-schooled students and high school students from underrepresented groups, including women and African-American students, in western North Carolina. Lastly, the new TEM will enhance service capabilities of the DMF for the surrounding communities and further strengthen collaborations among biologists, physicists, and other scientists, as well as faculty and students at other universities in western North Carolina. The project outcomes are available for public viewing on a dedicated page at the DMF website (http://www.casmifa.appstate.edu/index.html).