MRI: Acquisition of massively deep-read sequencing technology at NCSU

该奖项是根据2009年美国复苏和再投资法案(公法111-5)资助的。北卡罗来纳州立大学已获得一笔赠款，用于购买下一代“深度阅读”测序仪。该机器每次运行产生超过200亿个碱基的核苷酸序列，形式为超过4亿个50-72个碱基序列片段。它是正在进行的研究中必不可少的工具，需要：1)全基因组和靶向重测序；2)多态检测；3)发现小RNA；4)转录图谱。新设备将由NCSU的基因组科学实验室操作，并将服务于不同的用户社区，他们有兴趣了解生物体的基因型和其表型之间的联系，并识别在生存和繁殖方面存在个体差异的变异。该设备将把正在进行的研究转变为：1)微生物群落是如何构成的；2)复杂性状的遗传基础；3)生物多样性的起源和维持。在购买新设备的同时，还建立了核心生物信息学支持，以加快整个NCSU更大的研究社区向新技术的过渡。此外，这些设备(和由此产生的数据)将被广泛纳入本科和研究生课程，并将加强现有的i)基因组科学、生物信息学和生物技术方面的研究生和本科培训计划，ii)少数族裔招生计划，包括NCSU资助的研究生教育联盟和教授计划，以及iii)社区外展计划，如NCSU生物技术和生命科学暑期学院。这种新设备以及相关的培训和教育举措有助于确保NSCU保持在基因组科学的前沿。NCSU测序项目的结果将通过公共数据库、学生和教职员工在地区或国家会议上的陈述以及同行评议的期刊出版物来传播。

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).A grant has been awarded to North Carolina State University to purchase a next generation "deep-read" sequencer. The machine generates over 20 billion bases of nucleotide sequence per run in the form of more than 400 million 50-72 base sequence fragments. It is an essential tool in ongoing research requiring i) full genome and targeted resequencing, ii) polymorphism detection, iii) small RNA discovery, and iv) transcriptional profiling. The new equipment will be operated by NCSU's Genomic Science Laboratory and will serve a diverse user community interested in understanding the connections between an organism's genotype and its phenotype, and for identifying the variation that underlies individual differences in survival and reproduction. The equipment will transform ongoing research into i) how microbial communities are structured, ii) the genetic basis of complex traits, and iii) the origins and maintenance of biodiversity. The acquisition of the new equipment is coupled with the establishment of core bioinformatics support to accelerate the transition to the new technology throughout the larger research community at NCSU. Moreover, the equipment (and data generated from it) will be broadly incorporated into undergraduate and graduate curriculum and will strengthen established i) graduate and undergraduate training programs in Genome Science, Bioinformatics, and Biotechnology, ii) minority recruitment initiatives, including NCSU's NSF-funded Alliances for Graduate Education and the Professoriate program, and iii) community outreach programs such as NCSU Summer College in Biotechnology and Life Sciences. This new equipment and the associated training and educational initiatives help ensure that NSCU remains at the forefront of genomic science. Results from the NCSU sequencing projects will be disseminated through public databases, student and faculty presentations at regional or national meetings, and through publication of peer-reviewed journal publications.