Hybrid Bionanomechanical Systems for Single Molecule Detection

用于单分子检测的混合生物-纳米机械系统cagri Savran，普渡大学该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。摘要本研究的目的是开发一种具有单分子检测灵敏度的新型纳米机械传感器。该方法是在其尖端形成一个功能病毒的混合悬臂。这种悬臂/病毒混合结构将被用作鱼竿，用来捕获DNA，而DNA的另一端将通过抗体从样本中捕获目标实体。在捕获目标分析物时，DNA的包装和卷绕将通过与捕获的分子结合的磁珠使用磁驱动进行检测。智力优势：开发的系统将是第一个利用病毒活动实现以下目标的传感器：单分子敏感性，高效的目标捕获（目标捕获抗体不会固定在表面上，而是通过DNA延伸到溶液中）和可忽略不计的非特异性结合（只有病毒所在的悬臂的尖端会与溶液接触），因此最终将使纳米机械传感器在生物研究和医学中发挥作用。广泛影响：该项目有可能产生极其敏感的生物传感器，可以渗透到临床和生物研究实验室，并服务于从疾病生物标志物检测到单分子水平上蛋白质-蛋白质相互作用的理解等众多目标。此外，作为该提案的一部分，本科课程的实验室部分将被修改为具有MEMS/NEMS器件，因此将在未来几年教育本科生这个新领域。

Hybrid Bio-Nanomechanical Systems for Single Molecule DetectionCagri Savran, Purdue UniversityThis award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).AbstractThe objective of this research is to develop a novel nanomechanical sensor with single molecule detection sensitivity. The approach is to form a hybrid cantilever with a functional virus at its tip. The cantilever/virus hybrid structure will be used as a fishing rod to reel in the DNA, whereby the other end of the DNA will capture a target entity from a sample by means of an antibody. The packaging and reeling of the DNA upon the capture of target analyte will be detected using magnetic actuation via a magnetic bead that binds to the captured molecule.Intellectual merit: The developed system will be the first sensor that uses a virus activity to achieve: single molecule sensitivity, efficient target capture (target-capturing antibody will not be immobilized on a surface, rather it will be extended into solution via DNA) and negligible nonspecific binding (only the tip of the cantilever where the virus is located will be in contact with the solution) and hence will finally allow nanomechanical sensors to be useful in biological research and medicine. Broad impact: This project has the potential to result in biosensors that are extremely sensitive which can penetrate into clinical and biological research laboratories and serve to numerous goals that range from disease biomarker detection to understanding of protein-protein interactions at the single molecule level. Also, as a part of this proposal, the laboratory portion of an undergraduate course will be modified to have MEMS/NEMS devices, and hence will educate undergraduates about this new field for years to come.