SynBERC BioFAB Facility

这个生物制造设施（BioFab）的主要目的是确保合成生物工程研究中心（SynBERC）现有和未来的所有驱动试验台应用都能快速获得高质量的标准生物部件以及DNA合成和部件组装服务。两个重要的次要目的是：(1)开发和测试运营和扩展生产设施的最佳组织架构，该设施能够生产许多标准生物部件，并支持学术界和工业界更广泛的社区参与；(2)确保synberc开发的所有基础技术，从快速基因组规模的底盘再造到设备的功能组成标准，都可以广泛访问并迅速转化为SynBERC测试平台和工业。实际上，该设施将在启动时开展三个具体项目。具体来说，BioFab的工作人员将1)快速构建synberc指定的工程遗传系统原型，以实现测试平台驱动的目标；2)设计、构建和测试6000个新的BioBrick部件集合，用于控制大肠杆菌和啤酒链球菌的复制、转录、RNA加工和降解、翻译和蛋白质降解；3)与工业和学术合作伙伴合作，开发改进的工具，以支持设计、构建、以及由标准生物部件组装而成的工程基因系统的表征/测试。智力价值：世界的成功开发、推出和运营？美国首次设计并建造了制造高质量标准生物部件的设备，这将标志着生物学作为工程基板发展的重要转变。在学术界和工业界，生物实验室将标志着从个人工艺到工业的转变。以生产基因试剂为基础，有了较为成熟的工程技术平台。反过来，这将使生物工程更容易、更可预测，在能源、人类健康、信息处理等领域有潜在的应用。更广泛的影响：这是首个此类设施，对学术研究，教育，工业和全球产生了重大的更广泛的影响。BioFab设施将加速生物标准的采用和发展，同时扩展SynBERC？为更广泛的工业和学术界提供前沿研究。这个由专业人员组成和操作的零件工程设施将成为工业界和学术界定义和颁布标准的重要资源，从技术到专业实践。在教育中，工程专业的学生将首次有机会接触到用于控制基本但必要的细胞过程的改进的生物部件集合，使他们能够减少对技术问题的关注，更多地关注如何通过工程生物学来解决世界问题。年代问题。

ABSTRACT0946510, Arkin, University of California-BerkeleyThe primary purpose of this bio-fabrication facility (BioFab) is to ensure that all of the driving testbed applications of the Synthetic Biology Engineering Research Center (SynBERC) that are both existing and future have rapid access to high-quality standard biological parts as well as DNA synthesis and part assembly services. Two critical secondary purposes are: (1) the development and testing of the best organizational architecture for operating and scaling a production facility that is capable of producing many standard biological parts and that supports broader community participation across both academia and industry, and (2) ensuring that all foundational SynBERC-developed technologies, from rapid genome-scale reengineering of chassis to function composition standards for devices are broadly accessible and rapidly translated into SynBERC testbeds and to industry. Practically, the facility will pursue three specific projects at launch. Specifically, the BioFab staff will 1) rapidly prototype SynBERC-specified engineered genetic systems to achieve testbed-driven objectives, 2) design, construct, and test a collection of 6,000 new BioBrick parts for controlling replication, transcription, RNA processing and degradation, translation, and protein degradation in E.coli and S. cerevisiae, and 3) work in partnership with industrial and academic partners to develop improved tools for supporting the design, construction, and characterization/testing of engineered genetic systems assembled from standard biological parts. Intellectual merit: The successful development, launch, and operation of the world?s first design and build facility for making high-quality standard biological parts will mark an important transition in the development of biology as a substrate for engineering. Within academia and industry, the BioFab will mark a transition from individual ?crafts? based production of genetic reagents to a more mature engineering technology platform. In turn, this will make the engineering of biology easier and more predictable, with potential applications in energy, human health, information processing, and more. Broader impacts: This first-of-its-kind facility has significant broader impacts for academic research, education, industry, and globally. The BioFab facility will accelerate the adoption and development of biological standards while extending the SynBERC?s cutting-edge research to the broader industrial and academic communities. This professionally staffed and operated parts engineering facility will serve as an important resource for industry and academia for defining and promulgating standards, from technical to professional practice. Within education, engineering students will have access for the first time to improved collections of biological parts for controlling basic but essential cellular processes, allowing them to focus less on technical issues and more on identifying ways to engineer biology to solve the world?s problems.