Development of a Synchrotron Beamline for High-resolution Microdiffraction Analysis of Biological Macromolecules

该奖项由2009年《美国复苏与再投资法案》(公法111-5)资助。这一重大研究仪器-复苏与再投资(MRI-R2)奖项资助纽约结构生物学中心(NYSBC)用于生物大分子高分辨率微衍射分析的同步加速器光束线的开发。NYSBC是由十家纽约机构组成的联盟，提供支持结构生物学的基础设施。NYSBC的科学家们正在国家同步加速器光源(NSLS)的一种新的波动源(X5)上开发同步光束线，以支持NYSBC社区的当前需求，并预测NSLS-II的新波动源将提供的机会。显微衍射是新光束线的定义能力，这是NYSBC科学家和其他生物结晶学家对这一特性的高要求。新的光束线提供高度聚焦的X射线束，用于各种生物大分子的结晶学分析，包括酶、膜蛋白、DNA、RNA和多个大分子组分的组合，并且基于一种新颖的单色仪设计，该设计将波动器辐射聚焦到具有出色能量分辨率的稳定微束中，以优化异常散射相位实验。纽约州立大学附属实验室培训了数百名研究生和博士后实习生，其中许多人将受益于在他们的研究中使用新的光束线。更广泛的影响包括对将使用这种设备的学生和研究员的培训，以及新光束线实验所取得的基本发现的社会影响。此外，该项目的创新使与此开发相关的行业受益。由新设备实现的研究结果通过学生和教职员工在地区和国家会议上的陈述以及通过在同行评议的期刊上发表来传播。

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).This Major Research Instrumentation-Recovery and Reinvestment (MRI-R2) award funds the development of a synchrotron beamline for high-resolution microdiffraction analysis of biological macromolecules at the New York Structural Biology Center (NYSBC). The NYSBC is a consortium of ten New York institutions that provides infrastructure to support structural biology. NYSBC scientists are developing the synchrotron beamline on a new undulator source (X5) at the National Synchrotron Light Source (NSLS) to support current needs of the NYSBC community and to anticipate opportunities that new undulators at NSLS-II will provide. Microdiffraction is the defining capability of the new beamline, and this is a property in high demand by NYSBC scientists and other biological crystallographers. The new beamline provides highly focused x-ray beams for use in crystallographic analyses of a wide range of biological macromolecules, including enzymes, membrane proteins, DNA, RNA and assemblages of multiple macromolecular components, and is based on a novel monochromator design that focuses the undulator radiation into stable microbeams with exceptional energy resolution for optimization of anomalous scattering phasing experiments. NYSBC-affiliated laboratories train hundreds of graduate students and postdoctoral trainees, and many of them will benefit from using the new beamline in their research. The broader impacts include the training of students and fellows who will use this equipment and the societal impacts of basic discoveries made from experiments at the new beamline. In addition, innovations made in this project benefit industries associated with this development. Results from the studies enabled by the new equipment are disseminated by student and faculty presentations at regional and national meetings, and through publication in peer-reviewed journals.