Biotechnology of Supramolecular Complexes of Pyrene-Based Fluorescent Dyes

弗吉尼亚联邦大学化学系的弗拉基米尔·西多罗夫博士和同事们已经确定了一个基于环戊二烯的水溶性阴离子染料受体家族。 在NSF化学部大分子，超分子和纳米化学计划的支持下，Sidorov小组正在开发基于环戊二烯受体的技术，用于监测生物膜过程。 将设计和合成结合阴离子染料和与生物相关底物缀合的染料的基于环素的受体。 基于这些受体的技术将被开发用于膜渗漏检测、脂质体内容物的细胞内运输、药物递送系统的分析以及肝素寡聚体的分离。这项研究采用超分子化学技术来监测生物膜过程和分离生物活性化合物，将对人类治疗产生影响，并广泛影响生物有机和生物界。 该项目的研究、培训和教育活动将有助于人力资源开发。

Dr. Vladimir Sidorov and coworkers at the Chemistry Department of Virginia Commonwealth University have identified a family of cyclen-based receptors for water-soluble anionic dyes. With the support from the Macromolecular, Supramolecular, and Nanochemistry Program in the Division of Chemistry at NSF, the Sidorov group is developing techniques based on cyclen-based receptors for monitoring biomembrane processes. Cyclen-based receptors that bind anionic dyes and dyes conjugated to biologically-relevant substrates will be designed and synthesized. Techniques based on these receptors will be developed for membrane leakage assay, intra-cellular trafficking of the liposomal content, the analysis of drug delivery systems, and the separation of heparin oligomers.This research, which employs supramolecular chemistry in techniques for monitoring biomembrane processes and for separating bioactive compounds, would have implications for human therapeutics and broadly impact the bioorganic and biological communities. The research training and educational activities of this project would contribute to human resource development.