MRI: Acquisition of a Multifrequency Phase and Modulation Fluorimeter

该主要研究仪器奖资助肯塔基州大学结构生物学中心购买多频互相关相位和调制荧光计。 荧光计用于研究蛋白质和其他生物大分子的结构、稳定性和相互作用。 将使用荧光计进行的主要实验包括荧光共振能量转移，提供有关附着在生物分子上的荧光团之间距离分布的信息，以及各向异性（偏振），以研究生物分子之间的相互作用。 这些研究中感兴趣的一些分子包括神经溶解素、神经肽酶和硝基还原酶。 此外，该仪器可供其他区域机构的研究人员使用，并用于肯塔基州大学的年度生物荧光研讨会。 这些研究和教学工作的结果将通过摘要和同行评议的出版物，以及学生和教师在专业会议的积极参与广泛传播。

This Major Research Instrumentation award funds the acquisition of a multifrequency cross-correlation phase and modulation fluorimeter at the University of Kentucky Center for Structural Biology. The fluorimeter is used to study the structure, stability and interactions of proteins and other biological macromolecules. Major experiments that will be performed with the fluorimeter include fluorescence resonance energy transfer, providing information on the distribution of distances between fluorophores attached to biomolecules, and anisotropy (polarization), to study interactions between biomolecules. Some of the molecules of interest in these studies include neurolysin, neuropeptidase, and nitroreductase. In addition, the instrument is available for use by investigators at other regional institutions and used in the annual Biofluorescence Symposium at the University of Kentucky. The results of these research and teaching efforts will be broadly disseminated through abstracts and peer reviewed publications, as well as by active participation of students and faculty at professional meetings.