Role of thrombospondins for leukocyte trafficking to inflamed tissue (B03)

在这个项目中，我们将阐明血小板反应蛋白(TSPs)在髓系白细胞向炎症组织运输中的作用。为此，我们将首先通过质谱学确定这些基质细胞蛋白的相互作用组。随后，我们将使用先进的体外/体外测试和体内显微镜技术来表征TSPs对血管内相互作用以及对中性粒细胞和单核细胞血管外运动的影响。最后，我们将使用转基因小鼠来评估TSPs在实验性脓毒症中与这些免疫细胞运输的功能相关性。

In this project, we will elucidate the role of thrombospondins (TSPs) for myeloid leukocyte trafficking to inflamed tissue. To this end, we will first define the interactome of these matricellular proteins by mass spectrometry. Subsequently, we will employ advanced in vitro/ex vivo assays and in vivo microscopy techniques to characterize the effect of TSPs on intravascular interactions as well as on the extravascular locomotion of neutrophils and monocytes. Finally, we will use genetically altered mice to evaluate the functional relevance of TSPs for the trafficking of these immune cells in experimental sepsis.