Regulation of apical shoot meristem maintenance in arabidopsis thaliana by the newly identified gene FLAT SHOOT APEX (FSA).

分生组织由位于芽和根的顶端的少量形态上未分化的分裂细胞组成。萌发后，植物的所有气生部分都来源于茎顶端分生组织（SAM）。连续生长由SAM通过平衡中心未分化细胞的维持和外围细胞分化来控制。已经鉴定出控制分生组织功能的几个基因，包括WUSCHEL和CLAVATA基因。该提案旨在了解一种名为FLAT SHOOT APEX（FSA）的新分生组织基因的功能，该基因是在发育过程中维持适当SAM所必需的。本项目的目标是分析FSA在分生组织维持中的功能，并利用遗传和分子方法将FSA依赖的途径整合到现有的模型中，包括野生型和突变体背景下的表达分析，有丝分裂活动的分析，以及相互作用蛋白的分离。FSA在细胞分化和细胞分裂中的作用将得到解决。

Meristems comprise a small number of morphologically undifferentiated dividing cells located at the tips of shoots and roots. After germination all the aerial parts of the plant are derived from the shoot apical meristem (SAM). Continuous growth is governed by the SAM by balancing the maintenance of undifferentiated cells in the centre and the cell differentiation in the periphery. Several genes including WUSCHEL and the CLAVATA genes have been identified that control meristem function. This proposal aims to understand the function of a new meristem gene called FLAT SHOOT APEX (FSA), which is required to maintain a proper SAM during development. The goal of this project is to analyse the function of the FSA in meristem maintenance and to integrate the FSA-dependent pathway in the existing models using genetic and molecular methods including the expression analysis in wild type and mutant backgrounds, the analysis of mitotic activities, and the isolation of interacting proteins. The role of the FSA in cell differentiation and cell division will be addressed.