Multidimensional "omics" characterization of microbial metabolism and dissolved organic matter in Antarctica

揭示溶解有机物（DOM）的动态对于理解全球碳循环至关重要，因为来自湖泊，河流，海洋和土壤的有机物质会通过这个池。DOM是许多生态系统中微生物的重要能量来源，因此可以影响区域养分循环模式。例如，初步结果表明，从南极洲棉花冰川的冰上流中分离出的生物可能在这个相对简单的低温系统中对DOM循环很重要。然而，人们对环境中与DOM相互作用的微生物的功能属性知之甚少。该项目将使用最先进的基因组学，蛋白质组学和代谢组学方法来了解两种微生物分离物CG 3和CG9_1影响DOM循环的机制。液相色谱-质谱法也将用于更好地表征该生态系统中微生物来源的DOM。该项目将支持一名本科生和两名研究生的研究和培训。 将通过出版物以及在国家和国际会议上的发言广泛传播成果。此外，将通过开放式数据库提供原始数据。了解冷适应微生物代谢和DOM池之间的关系很重要，因为地球上90%以上的DOM池都是冷适应微生物代谢的产物。海洋温度低于5摄氏度。

Uncovering the dynamics of dissolved organic matter (DOM) is central to an understanding of the global carbon cycle, as organic material from lakes, streams, oceans and soils passes through this pool. DOM acts as a key energy source for microbes in many ecosystems and therefore can affect regional nutrient cycling patterns. For example, preliminary results suggest that organisms isolated from a supraglacial stream on Cotton Glacier, Antarctica, may be important in DOM cycling in this relatively simple, low temperature system. However, little is known about the functional attributes of the microbes that interact with DOM in the environment. This project will use state-of-the-art genomics, proteomics and metabolomics approaches to understand the mechanisms by which two microbial isolates, CG3 and CG9_1, affect DOM cycling. Liquid chromatography-mass spectrometry will also be used to better characterize the microbially-derived DOM from this ecosystem. This project will support the research and training of one undergraduate and two graduate students. Results will be widely disseminated through publications as well as through presentations at national and international meetings. In addition, raw data will be made available through open-access databases. Understanding the relationship between cold-adapted microbial metabolisms and DOM pools is important as more than 90% of the Earth?s oceans are below 5 degrees Celsius.