MRI: Acquisition of an ABI genetic analyzer for Research and Research Training at a Predominately Undergraduate Institution

应用生物系统310基因分析仪将为研究人员提供仪器，以促进和促进个体和合作研究机会的进步，在基因组学，分子进化生物学和系统，细胞和分子生物学，微生物学和法医学的动态和不断增长的领域在芬德利大学。基因分析仪将提供机会，以加强教师和本科生研究人员目前的研究，并提供令人兴奋的机会，以开发和实施新的研究思路和技术，可能只有通过先进的测序和片段生成和分析。需要广泛使用遗传分析仪的具体项目包括：1)使用序列和片段数据的进化生物学，包括两栖动物和爬行动物的系统地理学和种群遗传学；2)染色体的突变频率和结构重排；3)俄亥俄州北部自然保护区和恢复湿地中Ranavirus的发现和流行；4)商业和有机农场使用生物膜的抗生素耐药性；5)蛋白质内在紊乱的计算机和体外分析。作为一个以本科生为主的机构，仪器，如所要求的基因分析仪，在帮助我们将研究整合到我们的课程中至关重要，在我们的课程中，我们培养下一代科学家和培养教师。研究兴趣。提议的活动对芬德雷大学的教师和本科生研究人员、科学界和社会的广泛影响与NSF-MRI计划的目标一致，包括：1)通过培训课程为教师和更广泛的科学界提供共享科学仪器的研究和培训；2)开发跨学科和合作研究的机会；3)增加基础研究技术（如实验设计、分子方法和技术）以及与现代测序技术和片段分析相关的教学、培训和学习的机会；4)通过在科学期刊上发表同行评议的文章，出席科学会议并在会议上发言，向更广泛的科学研究界传播新的研究成果、方法和培训机会；5)为本科生提供使用先进仪器的特殊机会和实践培训，这些学生很少或只有在研究生阶段学习时才能接触到这些设备；6)培养批判性思维和解决问题的能力，增加本科生的学习经验；7)为该地区的中小学和大学生提供拓展机会，向他们感兴趣的领域的研究科学家学习基本的测序技术。

The Applied Biosystems 310 Genetic Analyzer will provide researchers with instrumentation to promote and foster the advancement of individual and collaborative research opportunities in the dynamic and growing areas of genomics, molecular evolutionary biology and systematic, cell and molecular biology, microbiology, and forensic science at The University of Findlay. The genetic analyzer will provide opportunities to enhance the current research of faculty and undergraduate researchers, and provide exciting opportunities to develop and implement new research ideas and techniques possible only through advanced sequencing and fragment generation and analysis. Specific projects that will require extensive use of the genetic analyzer include investigations into: 1) evolutionary biology using sequence and fragment data, including phylogeography and population genetics, of amphibians and reptiles; 2) mutation frequencies and structural rearrangements of chromosomes; 3) the discovery and prevalence of Ranavirus in a natural preserve and a restored wetland in northern Ohio; 4) antibiotic resistance using bioflims in commercial and organic farms; and 5) in silico and in vitro Analysis of Protein Intrinsic Disorder. As a predominantly undergraduate institution, instrumentation, such as the requested Genetic Analyzer, is crucial in assisting us to integrate research into our curriculum where we train the next generation of scientists and foster faculty?s research interests.The broader impacts of the proposed activities for the faculty and undergraduate researchers at The University of Findlay, the scientific community, and society are on par with the goals of the NSF-MRI program and include: 1) providing access to shared scientific instrumentation for research and training of faculty and to the broader scientific community through training sessions; 2) developing interdisciplinary and collaborative research opportunities among researchers; 3) enhancing opportunities for basic research techniques (e.g., experimental design, molecular methodologies and techniques), as well as teaching, training, and learning related to modern sequencing techniques and fragment analysis; 4) disseminating novel research findings, methods, and training opportunities to the broader scientific research community through peer-reviewed publications in scientific journals and attendance and presentations at scientific meetings; 5) providing exceptional access and hands-on training using advanced instrumentation to undergraduate students, who encounter such equipment rarely or only upon study at the graduate level; 6) developing critical thinking and problem-solving skills, which augments the learning experiences for undergraduate students; and 7) offering outreach opportunities for area primary, middle, and secondary schools, as well as area college students, to learn basic sequencing techniques from research scientists in their field of interest.