Generation and design of bifunctional bacteriophages and peptides: integrating organics and inorganics into multifunctional materials

在过去的几年里，对无机功能材料的需求强劲增长，目前的重点是不同特性的多功能复合材料。然而，由于技术上的困难，以及通过传统合成方法通过材料组装来融合几种性质的要求，纳米材料的生产受到限制。利用生物矿化来处理下一代材料提供了一种创新的解决方案，以克服传统处理方法的局限性。在拟议的项目中，计划制备具有导电性和热学性能的生物有机-无机材料(例如电容器和压电元件)。在我们的部分中，我们将通过基因工程将双功能噬菌体和多肽作为构建和组织这些材料组件的支架。将从噬菌体文库中鉴定和使用能够识别和启动具有前体盐溶液的无机相的受控矿化的多肽。利用生物技术和生物信息学相结合的方法，研究多肽识别和结合无机物的分子机制，设计出亲和力和特异性提高的多肽，筛选出的多肽与工程多肽的结合，无论是作为展示在噬菌体表面的融合蛋白，还是作为双功能多肽，都将有助于合成复杂的多功能复合材料。

The demand for inorganic functional materials has strongly increased over the last couple of years, with the focus now on multifunctional composite materials of different characteristics. However, the production of nanomaterials due to technical difficulties combined with the requirements for the fusion of several properties through material assembly by conventional synthesis methods is limited. The use of biomineralization to process the next generation of materials provides an innovative solution to overcome the limitations of traditional processing methods.In the proposed project it is planned to generate bioorganic-inorganic materials with electrical conductivity and thermal properties (e.g. capacitors and piezoelectrical elements). In our part we will genetically engineer bifunctional bacteriophages and peptides as scaffolds for constructing and organizing these material components. Peptides which can specifically recognize and initiate a controlled mineralization of an inorganic phase with precursor salt solutions will be identified from phage libraries and employed. Using an integrated approach based on biotechnology and bioinformatics, the molecular mechanism of peptide recognition of and binding to inorganic materials will be investigated and peptides with improved affinity and specificity will be designed.The combination of selected and engineered peptides, either as fusion proteins displayed on the phage surface or as bifunctional peptides, will facilitate the synthesis of complex multifunctional composite materials.