Deciphering subsurface microbial activity on the Iberian Margin

这项提议试图确定在IODP第339航次取心的海底沉积物中发现的微生物的特征，并将它们与秘鲁边缘附近类似沉积物中发现的特征良好的微生物组进行比较。元转录分析通过靶向整个样品信使RNA并将RNA与已知基因功能相关联来识别活性生物群。因此，可以评估整个微生物群落的代谢行为和分类，并确定活跃和非活跃生物群(古生物群和生物群)的相对比例。用于后转录分析的样本选自取自1的部分核心。)浅层次表层硫酸盐富集区；来自更深的地下的富含甲烷的样品，以及3。从硫酸盐-甲烷转变附近采集的样品。需要检验的假设是，这些化学变化与样本生物学直接相关。为了充分实现探险339号的目标，这项工作是必要的。更广泛的影响包括毕业生和早期职业调查员的参与、社区外联和IODP讲课。科学影响包括描述世界的一部分？S最大、最鲜为人知的生态系统，海底以下深层生物圈。发现新物种和代谢途径的潜力是巨大的。

This proposal seeks to characterize the microbes recovered from sub ocean floor sediments cored during IODP leg 339 and compare them to the well-characterized microbial suite found in similar sediments off the Peru margin. Metatranscriptomic analysis enables the recognition of the active biome by targeting the whole sample messenger RNA and relating the RNA to known gene function. Thus the metabolic behavior and taxonomy of the entire microbial community can be assessed and the relative proportion of active vs. inactive biota (paleome vs. biome) determined. The samples for metatranscriptomic analysis are selected from portions of the core taken from 1.) sulfate-rich areas in the shallow subsurface; 2.) methane-rich samples from the deeper subsurface, and 3.) samples taken from near the sulfate-methane transition. The hypothesis to be tested is that these chemical variations are directly related to sample biology. This work is needed in order for the objectives of expedition 339 to be fully met. Broader impacts include graduate and early career investigator involvement, community outreach, and an IODP lectureship. Scientific impacts include characterization of part of the world?s largest and most poorly understood ecosystem, the sub-seafloor deep biosphere. The potential for discovering new species and metabolic pathways is significant.