EAGER: Profiling Dynamic Changes in Animal Circadian Clock Protein Networks

智力优势：EAGER项目的目标是通过使用基于质谱的蛋白质组学和果蝇作为模型来描述昼夜节律振荡蛋白compleesx和其他细胞机制之间的动态相互作用。这个项目是开发基础数据来探测生物计时所需的昼夜节律相互作用组的动态重新布线。 展望未来，该项目的结果将为（i）新时钟组件的功能表征和（ii）具有不同时钟设计的多个物种的昼夜节律相互作用组的比较蛋白质网络分析奠定基础。这种比较加上严格的数学方法将突出网络重新布线和差分相互作用，可以反映固有的灵活性和适应性的时钟网络。更广泛的影响：昼夜节律生物学是一个迷人的话题，所有年龄和背景的人都可以涉及，因为昼夜节律时钟调节生理过程是许多人感兴趣的，因此代表了一个完美的科学教育平台。PI和她的同事们制定了一个多方面的计划，利用昆虫的昼夜节律来教育和激励未来各年龄段的科学家，并吸引公众的参与。该项目还将为研究生、本科生和高中教师提供跨学科培训和职业发展机会。

Intellectual Merit:The objective of this EAGER project is to profile the dynamic interactions between circadian oscillator protein compleesx and other cellular machineries by using mass spectrometry-based proteomics and Drosophila melanogaster as a model. This project is to develop foundational data to probe the dynamic rewiring of the circadian interactome necessary for biological timekeeping. Looking ahead, results from this project will lay the foundation for (i) functional characterization of new clock components, and (ii) comparative protein network analysis of the circadian interactomes in multiple species with distinct clock designs. This comparison coupled with rigorous mathematical approaches will highlight network rewiring and differential interactions that can reflect inherent flexibility and adaptability of the clock network.Broader Impacts:Circadian biology is a fascinating topic that people of all ages and backgrounds can relate to, as circadian clocks regulate physiological processes that are of interest to many, and therefore represents a perfect platform for science education. The PI and her colleagues developed a multi-faceted plan to use insect circadian rhythms to educate and inspire future generations of scientists of all ages and engage the general public. This project will also provide cross-disciplinary training and career development opportunities for graduate students, undergraduates, and high school teachers.