I-Corps: Commercialization of Immobilized Metal Affinity Chromatography Resins Based on Nanomaterials

提出了一种基于氧化石墨烯（GO）纳米片的新型固定化金属亲和层析（IMAC）树脂，用于蛋白质纯化。这些IMAC树脂可能会在蛋白质纯化市场上形成一种颠覆性的技术，远远超出目前IMAC固定相材料的应用水平。与市售树脂相比，这种新型IMAC材料有望提供更好的蛋白质装载能力和特异性。根据客户对IMAC材料及其可能衍生产品的需求，将探索其他应用。拟议的IMAC树脂最有前途的商业应用将根据客户发现和商业发展努力，通过生物技术部门的目标市场探索来确定。IMAC法是一种简便有效的分离高纯度蛋白质的方法。该团队估计，IMAC树脂全球市场可能会进一步增长。与现有的商业产品相比，本文提出的IMAC树脂将：a)具有更高的体积和重量金属密度，b)具有优越的蛋白质负载能力和纯度，c)确保最先进的耐久性和化学稳定性，d)促进分离蛋白质的高活性保留，e)提供结合亲和力与分析物蛋白质特异性的控制。在进行研究的过程中，对其中一些特征进行了调查。在IMAC树脂的自然业务领域之外，可能还有其他应用。

A new family of innovative immobilized metal affinity chromatography (IMAC) resins based on graphene oxide (GO) nanosheets for protein purification is proposed. These IMAC resins potentially constitute a disruptive technology in the protein purification market well beyond the current level of application of IMAC stationary phase materials. This novel IMAC material is expected to provide improved protein loading capacity and specificity compared to commercially available resins. Alternative applications in response to customer needs of the IMAC material and its possible derivatives will be explored. The most promising commercial applications of the proposed IMAC resins will be determined on the base of a customer discovery and business development effort by means of a targeted market exploration in the biotechnology sector.The IMAC method is an elegant and powerful approach for isolation of proteins with high purity. The team estimates that the IMAC resin global market is likely to experience further growth. The IMAC resin proposed herein, when compared to existing commercial products, will: a) be endowed with higher volumetric and gravimetric metal density, b) exhibit superior protein loading capacity and purity, c) ensure state-of-the-art durability and chemical stability, d) promote high retention of activity of isolated proteins, e) provide control of binding affinity versus analyte protein specificity. Some of these characteristics have been investigated in the course of the research conducted. Other applications may be possible beyond the natural business segment of the IMAC resin.