Collaborative Research: Inventories of Primary Productivity by In-situ Mass Spectrometry in the Upper Ocean

在海洋的大部分地区，从二氧化碳中产生有机物（特别是光合生物）和将有机物呼吸回二氧化碳（各种生物，包括光合生物）几乎处于平衡状态-但并非完全平衡。在一些远离陆地的地区，通过光合作用产生的碳比呼吸回二氧化碳的碳多;在其他地区，情况正好相反。 弄清楚这一点非常重要，因为在光合作用超过呼吸作用的地区，海洋生物的食物净生产以及二氧化碳（一种强大的温室气体）从大气中的净去除。 相反，呼吸作用超过光合作用的海洋区域是净食物消耗和向大气释放二氧化碳的区域。 在实践中，问题是，这种或那种差异是如此之小，以至于很难区分测量本身和数据中的噪声（即散射）。 解决这个问题需要新的技术，可以在海洋本身而不是在一瓶取样的海水中进行非常高精度的必要测量。 在这个项目中，调查人员将尝试使用通常只有在分析实验室中才能找到的复杂仪器-质谱仪，一种能够对溶解氧，二氧化碳和海洋水柱中与光合作用和呼吸作用有关的其他物质的量的变化进行超高精度测量的设备。 研究人员将支持研究生作为研究团队的组成成员参与。 此外，还将对中学生进行公共教育宣传，让他们有机会参与该项目，以获得第一手经验，了解如何利用基础科学解决海洋学问题。在该项目中，一组调查人员将使用原位质谱法评估这些方法的一个方面，以更好地限制净群落生产量（NCP）。UMIMS是一种快速响应的水下膜入口质谱仪，可部署在自主和远程操作的车辆中。在此应用中，UMIMS将被部署在SeaSoar拖曳车辆上，以制作超过氩气（Ar）预算的过量溶解氧（O2）的高分辨率垂直剖面，这是一种有前途的海洋表面NCP测量方法。配置文件与UMIMS可以提供的能力，以解决整个混合层和透光层的过程，影响NCP预算。在短期内，该团队将利用UMIMS的能力来制作精确和同步的O2和Ar混合层剖面，并将UMIMS的NCP测量结果与正在航行的海水中常规船上O2/Ar预算的测量结果进行比较。这些比较将被用来确定这两种方法的局限性，以及它们在多大程度上可以解决导致非稳态交换的环境物理过程。从长远来看，研究人员希望在拉格朗日浮子和海上滑翔机上部署UMIMS所需的技术和方法上取得进展。因此，该项目预计将朝着测量海洋代谢平衡和生物泵的近连续时间和空间系列观测迈出重要一步，类似于Argo浮标测量今天的温度和盐度的方式。

Production of organic matter from carbon dioxide (specifically by photosynthetic organisms) and respiration of organic matter back to carbon dioxide (by a wide variety of organisms, including photosynthesizers) are very nearly in balance over much of the ocean -- but not quite. In some regions remote from land, more carbon is produced through photosynthesis than is respired back to carbon dioxide; in others, just the opposite is true. Figuring out which is exceedingly important because in regions where photosynthesis exceeds respiration, there is a net production of food for marine life as well as a net removal of carbon dioxide (a powerful greenhouse gas) from the atmosphere. Conversely, oceanic regions where respiration exceeds photosynthesis are zones of net food consumption and release of carbon dioxide to the atmosphere. In practice, the problem is that the differences, one way or the other, are so small that it is difficult to distinguish between the measurements themselves and noise (that is, scatter) in the data. Resolution of this problem requires new technology that can make the necessary measurements with very high precision in the ocean itself rather than in a bottle of sampled seawater. In this project, the investigators will attempt to do just that using a sophisticated instrument normally found only in an analytical laboratory -- a mass spectrometer, a device capable of making ultra-high-precision measurements of changes in the amount of dissolved oxygen, carbon dioxide, and other substances associated with photosynthesis and respiration in the marine water column. The investigators will support graduate students to participate as integral members of the research team. There will also be public educational outreach to secondary school students offering them the opportunity to engage with the project to gain first-hand experience seeing how the basic sciences can be used to solve oceanographic problems.In this project, a team of investigators will evaluate one facet of these methodological concerns using in-situ mass spectrometry to better constrain net community production (NCP). The UMIMS is a fast-response Underwater Membrane Inlet Mass Spectrometer that can be deployed in autonomous and remotely-operated vehicles. In this application, UMIMS will be deployed aboard a SeaSoar tow vehicle to make high resolution vertical sections of the excess dissolved oxygen (O2) over argon (Ar) budget, a promising measure of NCP in the surface ocean. Profiles with the UMIMS can provide the capacity to resolve processes throughout the mixed-layer and euphotic zone that affect the NCP budget. In the near term, the team will utilize the capabilities of the UMIMS to make accurate and simultaneous mixed layer profiles of O2 and Ar and compare measurements of NCP from the UMIMS with measurements from the conventional shipboard O2/Ar budget from underway seawater. These comparisons will be used to determine the limitations of both methods and the degree to which they can resolve the ambient physical processes leading to non-stationary exchange. In the long term, the researchers expect to make the technological and methodological advances necessary to deploy the UMIMS on Lagrangian floats and sea gliders. As such, this project is expected to make a significant step toward measuring near-continuous time and space series observations of the oceanic metabolic balance and the biological pump, similar to the way Argo floats measure temperature and salinity today.