I-Corps: Commercialization of immobilized enzymes, processes and services

开发具有成本效益、增强酶活性和允许多次反应循环的固定化酶制剂需要专业知识。令人惊讶的是，大型酶生产商并没有组织起来提供这种能力。他们的重点是高效地生产酶，以满足大批量市场的需求。同样，很少有化学品制造商有内部能力开发高效的酶催化制造工艺。固定化酶生物催化剂系统的商业化开发有可能为化学工业提供变革性的新技术，降低过程能量投入，避免保护-去保护步骤。该团队在创造高性能固定化酶方面具有深刻的见解和实践经验。提出的技术将深入了解酶的性质，包括活性和稳定性，如何受到固定化树脂的表面化学和物理参数的影响。通过确定酶和固定化树脂的最佳组合，可以显著提高酶的活性和稳定性。拟议中的新公司还将开发树脂-酶组合的见解，以改善固定化酶生物催化剂的多循环使用。在开发高性能固定化酶系统中获得知识的关键途径将是实施高通量筛选过程。

Expertise is needed to develop immobilized enzyme preparations that are cost-effective, enhance enzyme activity and allow multiple reaction cycles. Surprisingly, large enzyme producers are not organized to provide this capability. Their focus is to efficiently manufacture enzymes that meet demands in high volume markets. Similarly, few chemical manufacturers have the internal capability to develop efficient enzyme catalyzed manufacturing processes. The commercial development of immobilized enzyme biocatalyst systems has the potential to provide transformational new technologies to the chemical industry that lower process energy input and avoid protection-deprotection steps. This team has developed deep insight and practical experience in creating superior performance immobilized enzymes. The proposed technology will develop a deep understanding of how enzyme properties, including activity and stability, are influenced by the surface chemistry and physical parameters of immobilization resins. By identifying optimal combinations of enzymes and immobilization resins, important improvements in enzyme activity and stability will be achieved. The proposed new company will also develop insights into resin-enzyme combinations that improve immobilized enzyme biocatalysts for multiple cycle use. A key approach to gain knowledge in the development of high performance immobilized enzyme systems will be the implementation of a high throughput screening process.