UNS:Photomodulation of Forster Cycle in a Fluorescent Protein

主要研究者：卡尔坎，卡恩提案编号：1512157该项目旨在开发新型的光学探针，其转换速度更快，效率更高，同时减少光漂白的影响。他们将研究金属纳米颗粒和荧光蛋白之间能量转移的物理学，以开发用于超分辨率显微镜应用的稳定的光激活探针。假设的光开关是基于使用表面增强光泵浦的FP的Forster循环中的中间态的光产生。将通过单分子表面增强拉曼散射和显微光学光谱来验证和研究福斯特循环的这种光调制。这项工作将开发一个光开关使用绿色荧光蛋白（GFP）-银NP共轭物作为其原型。光调制是基于通过激发态质子转移的GFP的中间形式（I-形式）的光致布居，所述激发态质子转移使光吸收从395 nm红移至490 nm。这种吸收的光谱偏移进而预期通过共振能量转移到FP来调制等离子体NP的米氏散射。

PI: Kalkan, KaanProposal No.: 1512157This project seeks to develop new types of optical probes that are faster and more efficient at switching, while reducing effects of photobleaching. They will study the photophysics of energy transfer between metal nanoparticles and fluorescent proteins to develop stable photoactivatable probes for super-resolution microscopy applications.The hypothesized photoswitching is based on photocreation of the intermediate state in the Forster cycle of the FP using surface-enhanced optical pumping. This photomodulation of the Forster cycle will be verified and studied by single molecule surface-enhanced Raman scattering and micro-optical spectroscopy. This effort will develop a photoswitch using Green Fluorescent Protein (GFP) - silver NP conjugates as its prototype. The photomodulation is based upon photopopulation of the intermediate form (I-form) of GFP by excited state proton transfer that red-shifts the optical absorption from 395 to 490 nm. This spectral shift in absorption, in turn, is expected to modulate the Mie scattering of the plasmonic NP by resonant energy transfer to the FP.