SBIR Phase I: Enhanced delivery of bioactive compounds for aquaculture

这一小型企业创新研究第一阶段项目的更广泛的影响/商业潜力将是改善水产养殖养殖的鱼和贝类的供应，同时改善在集约管理下养殖的鱼和贝类的福利。生物活性化合物或生物制品(如疫苗、药物和营养食品)的口服输送在水产养殖中具有挑战性，原因如下：1)制造过程中的热损害，2)饲料在储存过程中经常面临高温和潮湿，降低其生物活性，3)材料在水中运输，以及4)材料必须通过具有挑战性的条件在胃肠道中，然后定位效应部位。该项目开发口服给药和保护生物活性化合物和生物制品活性的配方和方法，用于保护动物免受疾病侵袭。该项目开发的方法将减少提供保护所需的这些材料的数量。由于生物活性化合物和生物制品是高成本材料，使用大大减少的材料量交付相同的活动将降低它们在水产养殖中的使用成本。在水产养殖中更有效地控制病原体和改善动物健康，因为更负担得起这些高价值化合物的应用，将提高水产养殖的可持续性。在这些配方中成功部署更多负担得起的生物制品将促进水产养殖生产，从而减少世界对野生捕捞渔业的依赖。这一第一阶段研究项目的技术目标集中在开发口服给药配方和方法，以改进寡糖、多糖和蛋白质的给药，这些寡糖、多糖和蛋白质可应用于对水产养殖业具有重要商业价值的其他生物活性化合物和生物制品。由于生产、储存和运输过程中的高湿度条件，水产养殖面临着有效运送高成本生物制品和生物活性化合物的困难环境。在第一阶段，S将专注于专利递送配方的成分优化，以提高和验证它们保护和递送不同类别化合物(寡糖、多糖和蛋白质)的能力。第一阶段的成功将完成给药系统的初步开发，并开始验证两种口服给药产品的免疫刺激能力，这两种口服给药产品的基础是已经显示出免疫刺激能力的寡核苷酸和游离β-葡聚糖产品的改进给药。

The broader impact/commercial potential of this Small Business Innovation Research Phase I project will be to improve the availability of aquaculture-raised fish and shellfish while improving their welfare when grown under intensive management. Oral delivery of bioactive compounds or biologics (such as vaccines, drugs and nutraceuticals) is challenging in aquaculture due to a number of factors: 1) heating damage during manufacturing, 2) feeds often face high temperature and humidity during storage that degrades their biological activity, 3) materials are delivered in water, and 4) materials must pass through challenging conditions in the gastrointestinal tract and then locate effector sites. This project develops formulations and methods to orally deliver and protect the activity of bioactive compounds and biologics, which are used to protect animals from disease. The methods developed in this project will reduce the amount of these materials necessary to provide protection. Since bioactive compounds and biologics are high cost materials, delivery of the same activity using a much reduced amount of the material will lower the cost for their use in aquaculture. More effective control of pathogens and improved animal health in aquaculture due to more affordable application of these high value compounds will improve aquaculture sustainability. Successful deployment of more affordable biologics in these formulations will be a boost to aquacultural production that can decrease the world's reliance on wild-catch fisheries. The technical objectives of this Phase I research project are focused on the development of oral delivery formulations and methods for improved delivery of oligoribonulceotides, polysaccharides and proteins that can be applied to other bioactive compounds and biologics of commercial importance for the aquaculture industry. Aquaculture presents a difficult environment to effectively deliver high cost biologics and bioactive compounds due to the high moisture conditions during manufacture, storage and delivery. During Phase I, the project?s focus will be on optimization of the components of proprietary delivery formulations to improve and validate their ability to protect and deliver different classes of compounds (oligoribonulceotides, polysaccharides, and proteins). A successful Phase I will complete the initial development of the delivery system and begin validation of the immunostimulatory abilities of two oral delivery products based on the improved delivery of oligoribonucleotides and free beta-glucan products that have already demonstrated immunostimulatory ability.